To understand the principles and methods of growth hormone induced adventitious root formation in plants through experiments.
Operation method
Experiments on the effect of growth hormone on the rooting of mung bean seedlings
Principle
Growth hormone not only promotes cell elongation and growth, but also promotes cell division and differentiation. Treatment of the hypocotyls of mung bean seedlings with appropriate concentrations of growth hormone can induce the occurrence of adventitious roots. Within a certain concentration range, the number of roots occurring is positively correlated with the growth hormone concentration.
Materials and Instruments
Mung bean seeds Move I. Materials, equipment and reagents For more product details, please visit Aladdin Scientific website.
IAA Master Mix
Light Incubator 100 ml volumetric flask 50 ml beaker Porcelain plate Scissors Wooden ruler
1. Material: mung bean seeds.
2. Equipment: light incubator; 100 ml volumetric flask; 50 ml beaker; porcelain plate; scissors; wooden ruler.
3. Reagent and preparation: 100μg-ml-1IAA mother liquor: accurately weigh 10mgIAA in a small beaker, add a few drops of 95 ﹪ ethanol to dissolve it and then dilute it with distilled water, then pour it into a 100 ml volumetric flask and make sure the volume is fixed to the scale.
Experimental steps
1. Cultivation of mung bean seedlings
Selected mung bean seeds, soak the seeds and sow them into a porcelain plate with sand, cover the seeds with sand, and cultivate them at about 25℃ in the light place until they grow two primary leaves and one heart leaf.
2. Series of IAA concentration solutions were prepared: 100 μg-ml-1 IAA mother solution was used to dilute into a series of 10, 1, 0.1, 0.01 μg-ml-1 concentration solutions.
3. Induction of mung bean seedling rooting culture
3.1 Sixty sand-cultivated mung bean seedlings (2 primordial leaves and one heart) of uniform growth were selected, and 3 cm hypocotyls were retained from the cotyledonary node, while the rest of the hypocotyls were excised together and the residual cotyledons were removed.
3.2 Six clean 50 ml beakers were taken, numbered, and 20 ml of IAA solution of 100, 10, 1, 0.1, 0.01 μg- ml-1 were added, and 20 ml of distilled water was added to the control (CK). Then 10 plants were placed in each treatment.
3.3 The treatments were incubated in a place with good light transmission to observe the rooting time and number of roots. (Always note that the level of culture solution in the beaker was basically constant, and if it was reduced due to evaporation, replenish it to the original level with distilled water.
Measurement results
After 10 days of incubation, measure the results according to the table below.
Effect of different growth hormone (IAA) concentration treatments on rooting of mung bean seedlings 