3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Oat, a temperate cereal crop widely grown worldwide, lacks tolerance to osmotic stress caused by drought and/or high salt. We have developed a genotype-independ
The study of insertion patterns and loci of exogenous genes in transgenic plants greatly contributes to the understanding of the mechanisms of integration, expr
Over the past 30 years, fluorescent chromosome analysis techniques have facilitated the understanding of genome construction and deepened knowledge of the organ
With the development of transgenic technology systems, the transfer of heterologous genes into the plant genome has become possible, which provides a new avenue
Agrobacterium-mediated transformation of barley has been widely utilized over the last decade and it offers advantages over gene gun-mediated transgenic techniq
In Western Europe, for transgenic crops to be approved for commercial production, proof of their substantial equivalence to conventionally bred crops needs to b
Within this chapter, we present a protocol for extracting total protein from wood-forming tissues (secondary xylem in differentiation). This protocol has been u
In this chapter we describe an efficient method suitable for whole protein extraction from various plant tissues. The method utilizes trichloroacetic acid and a
Plant cell mitochondria perform a number of important biochemical processes. The most important of these is the oxidative degradation of organic acids via the t
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