freehand sectioning experiments
Freehand sectioning is a method of holding a blade or razor and cutting fresh or fixed material into thin slices, which are then mounted and used for microscopi
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Plant paraffin sectioning technique
Due to their large size and opacity, plants cannot be viewed directly under a microscope. To study the internal structure of the plant, the thickness and volume
Determination of tannin content in sorghum grain
Tannins are generally considered to be water-soluble polyphenolic compounds with a molecular mass of 500-3,000 ku0 In addition to their phenolic properties, tan
TUNEL assay for programmed cell death in plants
Programmed cell death (PCD) is a genetically regulated process that actively removes excess, injured cells during plant growth and development or in response to
Detection of pollen viability and stigma pollinability
The pollination process begins with anther dehiscence and dispersal of mature pollen. Pollen grains carrying male gametes or their precursors are exposed to dry
Observations on pollen germination and pollen tube growth in vitro
After landing on the stigma, pollen, the male gametophyte of plants, grows pollen tubes through germination and transports sperm cells to the embryo sac to comp
Plant chromosome karyotyping
Various organisms in nature have relative stability in the form, structure and number of their chromosomes. The complete set of chromosomes contained in the gam
In vivo observation of the microfilament skeleton of plant cells
In a broad sense, the cytoskeleton is a type of organelle that contains three different components: microtubules, microfilaments, and intermediate fibers. So fa
Fluorescent labeling and observation of plant organelles
Plant cells contain abundant organelles, including vesicles, plastids, mitochondria, Golgi apparatus, ribosomes, endoplasmic reticulum, microtubules and microfi