Poly(A)+ RNA Preparation Experiments
The use of cND A microarray technology can be used to (1) analyze and detect the expression of thousands of genes in parallel; (2) play an important role in the
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
RNA extraction and labeling experiments
None
Chromosome preparation experiments on cultured peripheral blood cells
Although chromosomes can be prepared from other cells, human peripheral blood leukocytes are the easiest to synchronize, allowing high-resolution analysis of th
Plant RNA preparation experiment
Since there are many sources of RNA species, there are different methods of extraction and preparation, generally phenol method, detergent method and guanidine
In vitro splicing analysis experiments in mammalian cells
It is typical for splicing reactions to use nuclear extracts, i.e., S100 extracts complementing partially purified fractions of SR proteins or crude extracts, m
Cytoplasmic RNA extraction
Since there are many sources of RNA species, there are different methods of extraction and preparation, generally phenol method, detergent method and guanidine
Yeast Spliceosome Analysis Experiment
A precursor is a macromolecular ribonucleoprotein complex that splices mRNA precursors in the nucleus of eukaryotic cells. It consists of five snRNPs and a larg
Fungal RNA extraction
Fungal RNA extraction can be: (1) used in the study of fungal RNA interference mechanism; (2) used in cDNA library construction and other studies; (3) used in t
Northern blotting and narrow line hybridization analysis of RNA
Northern blot is one of the most rigorous methods to study gene expression, which can quantitatively analyze the expression abundance of a specific mRNA in tiss