Extraction of RNA from tissues by the CsCl method
Because some tissues (e.g., pancreas and uterus) contain high concentrations of endogenous RNAases, more care must be taken in purifying tissue-derived RNA.
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Effect of different methods of treating tissues on the yield of extracted RNA
RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)
Synthesize 5' capped transcripts
Some RNA transcripts require an m7 G(5')Ppp(5')G cap at the 5' end, and they have higher translational efficiency only in cell-free extracts or in African Xenop
RNA extraction from formalin-fixed, paraffin-embedded tissues
Formalin-fixed, paraffin-embedded tissues from the pathology department can be used for RNA expression analysis. RNA isolated from biopsy, surgical, and cadaver
Rapid isolation of total RNA from mammalian cells
SDS lyses the cells and extracts the total RNA by acid phenol graded extraction, which is a suitable method for treating cells with relatively low mRNA content
Extraction and isolation of rat liver rRNAs
Rat liver was homogenized, the supernatant was centrifuged, and RNA was extracted with a mixture of phenol and m-cresol. rRNA and mRNA were separated in the pre
nuclear transcription analysis
Nuclear runoff (runoff) analysis can be used to examine the transcriptional efficiency of specific genes in isolated cell nuclei. For runoff analysis, the nucle
Nuclease cleaves RNA at a specific site
Nuclease is a useful tool for obtaining site-specific cleavage products of long fragments of RNA for the study of RNA modification, for modification site analys
RNA reverse transcription
The RNA template is used to obtain DNA by reverse transcription, which is then used to replicate and infect cells.