mRNA 3' end processing in vitro
There are two strategies for in vitro experiments; one is to co-incubate a plasmid containing the target gene at the Po ly( A) locus with cell-free extracts to
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Primer extension method RNA analysis
The primer extension method can be used for RNA mapping and RNA 5' halo. In this method, an excess of a 5' end-labeled single-stranded RNA primer is hybridized
Methylmercury hydroxide agarose gel electrophoresis
Methylmercury hydroxide reacts with the imine bonds on uracil and guanine in RNA, which are involved in Watson-Crick base pairing. Therefore, methylmercury hydr
Spot and slit hybridization
Spot and slit hybridization analysis of RNA enables the qualitative or semi-quantitative determination of RNA by directly spotting RNA samples onto nitrocellulo
Extracellular system for translating viral mRNA
Translation of poliovirus and hepatitis C virus mRNA with mammalian cell S10 extracts.
RNA SI Nuclease Mapping
SI nuclease is an endonuclease that was isolated from Aspergillus oryzae. It can degrade single-stranded nucleic acids but not double-stranded nucleic acids. In
RNA spectroscopy and quantification
Nucleic acids are accurately quantified using spectrophotometry because this method does not destroy the structure and also allows for sample recovery. RNA has
A rapid response system for coupling in vitro transcription with in vitro translation
TNT 耦联网织红细胞裂解物系统乂可以分为：TNTT7 系统、TNTSP6 系统和 TN 下 PCR 系统。 The TNTT7 system translates proteins from both linear and circular DNA, the S
Glyoxal/DMSO denaturing agarose gel electrophoresis
Under slightly acidic conditions, the two acetaldehyde groups of glyoxal interact with the imino group of guanosine to form a cyclic compound, which inhibits th