3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
A bacterial anti-transcription termination detection system for studying peptide-RNA interactions by screening recombinant cDNA libraries. This experiment was d
Post-transcriptional gene silencing (PTGS) is known as RNA interference (RNAi), whereby double-stranded RNA (dsRNA) consisting of positive-sense and negative-se
Blot hybridization is the process of binding a nucleic acid fragment to be tested to a certain solid phase support and then hybridizing it with a labeled probe
Determination of DNA content can be used to (1) perform gene fragment ligation experiments. (2) Perform plasmid concentration determination prior to transformat
Plasmid preparation can be used for (1) carrying exogenous genes into bacteria for amplification or expression of the main vector, which has an important role i
In situ hybridization can be used for (1) solid-phase molecular hybridization and (2) labeled DNA or RNA as probes to detect specific nucleic acid sequences in
Denaturing gradient gel electrophoresis (DGGE) is a gel system that enables the separation of DNA fragments according to their melting properties. (1) By settin
DNA sequence analysis techniques can be used to (1) analyze the genetic diversity of species, (2) identify new species, and (3) for comparative genomics.
SNP (Single Nucleotide Polymorphism), or Single Nucleotide Polymorphism, can be used to detect nucleic acid sequence polymorphisms (Polymorphism) due to changes
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