Purification of expressed proteins from inclusion bodies
This experiment was mainly used to obtain soluble active proteins.
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Experimental purification of maltose-binding fusion proteins by affinity chromatography with straight-chain starch resin
Affinity chromatography methods can virtually purify maltose-binding fusion proteins into single components. Maltose-binding protein self (MBP) is a periplasmic
Lipofection-mediated DNA transfection assay
The following method is a modification of one studied by Mark Evans (Alexion Pharmaceuticals, New Haven, Connecticut). This experiment was derived from the next
Gel blocking assay of DNA binding proteins
The gel-blocking assay for analyzing DNA-binding proteins was invented by Fried and Crothers (1981) and was the first to provide a dynamic method for analyzing
Mapping of protein binding sites on DNA by DNAase I footprinting assay
This protocol describes a mapping method for identifying protein binding sites on radiolabeled DNA fragments. The protocol uses DNAase I to cut the DNA, whereas
DNA transfection experiment using Polybrene
The Polybrene and DMSO transfection method described below is a modification of the method of Aubin et al. (1997). Using this method, plasmid DNA can be efficie
DEAE-dextran-mediated high-efficiency transfection assay
Two forms of the classical DEAE-dextran transfection method are introduced here. The first one (the main option), in which the cells are exposed to high concent
Rapid analysis of λ phage isolates (purification of λ DNA from plate lysates) assay
Analysis of promising genomic or cDNA clones usually begins with digestion of small preparations of λ phage DNA with restriction enzymes and analysis of the di
DNAase I hypersensitive site mapping
DNAase I hypersensitive site mapping is frequently used to localize regulatory regions of eukaryotic genes. For reasons that are not yet understood, genes carry