Operating on an open workbench
Clean and wipe the work area, place the reagent bottles, pipette, etc., on the bench (Figure 6.5), and make preparations. If necessary. If necessary, flame ste
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Ultra-clean table aseptic operation method
Clean and wipe the work area, and then place bottles, pipettes, etc. in the work area. Then, start the preparatory work of preparing the culture medium and othe
Improvement of the Koch method of acute toxicity testing
1, study the design of acute toxicity test of chemicals, master the determination of LD50. 2. Observe the toxic reaction of phoxim.
Experiments on collection and preparation of biological materials for laboratory animals
The study of the toxic effects of foreign compounds. It is often necessary to determine the concentrations of compounds or their metabolites in the blood, urine
Handling of Petri dishes and plates
The manipulation of Petri plates is necessary for cell experiments such as (1) cell growth (2) cell propagation (3) cell inoculation.
Experimental purification of λ phage particles by precipitation/centrifugation
If the purpose of purification of the phage pellet is to obtain outgrown DNA fragments for subsequent subcloning or to prepare phage two-arms, centrifugation ca
Electrical activity of single neurons: the membrane clamp technique
Over the last 20 years, the membrane clamp technique has become one of the main research tools in modern electrophysiology. Initially used only to measure singl
Experiments on optical recording techniques for culturing single neurons
Techniques for optically recording the course of activity of individual viable neurons must be considered in the light of two fundamental questions, namely, wha
Restriction enzyme digestion labeling genome scanning for mutation detection experiments
Today, there is a need for more effective methods in the detection of mutations in humans and other organisms caused by environmental toxins and radiation expos