Optical recording experiments of population neurons in brain slices
Optical recording involves the use of molecular indicators whose optical properties (absorption or fluorescence) vary according to the parameters of cellular ac
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Analytical methods for correlating electrical muscle activity with animal locomotion
This chapter deals with two main aspects: (i) the methodology for simultaneous recording of EMG and video on normally moving animals, and (ii) the timing and ne
Human nerve fiber microelectrode recording experiment
The purpose of this chapter is first to introduce the technique to scientists who wish to use it in a practical way. Secondly, typical examples of the results o
magnetoencephalography
In physics, any electric current is accompanied by the production of a magnetic field. Ion currents flowing inside the brain and nerves also generate magnetic f
positron emission tomography (PET)
Positron emission tomography (PET) is an in vivo functional neuroimaging technique that provides three-dimensional quantitative images of a wide range of physio
Chemical and protein hydrolysis modification experiments of antibodies
This chapter is intended as a practical guide for researchers who need to modify antibodies themselves. It covers common chemical and protein hydrolysis modific
nuclear transplantation
Nuclear transplantation is based on the theory that all nuclei from the same embryo or cell line contain exactly the same genetic information as the fertilized
Human brain invasive techniques: microelectrode recording and microstimulation
The techniques discussed in this chapter allow recording under local anesthesia of the activity of cortical and subcortical structures such as the thalamus, bas
Preparation of pseudotyped lentiviral vectors resistant to complement inactivation
This experimental protocol describes several methods for generating complement-stabilized lentiviral vectors either by using fusion packaging proteins containin