Lentiviral transduction of hematopoietic cells
Most hematopoietic cells, including hematopoietic stem cells and terminally differentiated cells such as primary T cells and macrophages, are undifferentiated o
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3228 articles
Design method for adeno-associated heterotrimeric viral vectors
A A V coat peptide display library, error-prone P C R and D N A recombination . A A V serotypes with different histophilicity can be generated by natural mutati
Construction of first-generation adenoviral vectors
The possibility of immune responses against proteins encoded by viral vector backbones limits the application of transgene vectors for in vivo expression. Never
technology that targets genes
Gene pooping is an experimental tool for targeted alteration of genetic information of a species based on embryonic stem cells (ESCs) and homologous recombinati
Production and use of lentiviral vectors based on feline immunodeficiency virus
Lentiviral vectors based on feline immunodeficiency virus (F IV ) are very useful for mediating integrated transgenesis into non-dividing human cells. This chap
Reverse transcription virus vector assay
Reverse transcription viruses are well suited to be used as gene introduction tools because of their specific life cycle and ability to integrate efficiently in
Cyclodextrin-containing polycations for nucleic acid delivery
A large number of non-viral systems have been used for nucleic acid delivery to cultured cells in vitro or to specific cells in vivo. They vary considerably in
Reverse genetics of influenza viruses
It mainly describes the methods used to recover infectious influenza viruses from cDNA clones. These techniques are widely used in the integration and expressio
Polylysine copolymers for gene delivery
Polylysine and its copolymers have gained wide application as non-viral gene vectors. Although polylysine itself is toxic to cells, the covalent attachment of p