Solid lipid nanoparticles for in vitro mammalian cell transfection
Solid lipid nanoparticles (S L N ) have many technical advantages over gene carriers such as cationic liposomes and cationic polymers. However, in the absence o
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Targeted electrically neutral lipid vesicles for systemic delivery of genes
This chapter describes the detailed experimental procedure for encapsulation of nucleic acids by nanolipid particles (N L P ). This N L P carries a polyethylene
lipoplex and LPD nanoparticles for in vivo gene delivery
The core of gene therapy research lies in the development of suitable delivery systems. Although most clinical experiments currently utilize viral carrier syste
PEGylated nanoparticles of poly(L-lysine) and DNA
Nanoparticles of P E G-coated poly(L-lysine) and D N A are water-soluble, stable in saline and tissue fluids, transfect undivided cells (Liu et al. 2003), show
HVJ Liposomes and HVJ Packaging Vectors
This chapter describes the construction of a fusion-mediated vector based on inactivated H V J (hemagglutinating virus of Japan, also known as Sendaivirus). The
Transposon-mediated delivery of small interfering RNAs: the "Sleeping Beauty" transposon experiment
A method for efficient delivery of pol III-driven SHRNA expression frames using the Sleeping Beauty (SB) transposon. Advantages of this system include its ease
Cross-linked hyaluronic acid matrix and film experiments with specific plasmid DNAs
Natural polysaccharides are being extensively studied as carriers for gene delivery. The non-inflammatory and non-immunogenic nature of hyaluronic acid (H A ) i
Experiments on nanogene vectors based on polylactic acid and polyethylene glycol
Nanoparticles combining polylactic acid-hydroxyglycolic acid (PLGA) and polyethylene glycol (PEG) derivatives have been used as gene carriers for mucosal permea
Linear polyethyleneimine: synthesis and in vitro and in vivo transfection manipulation experiments
Linear polyethyleneimine (L P E I ) is a very effective transfection reagent suitable for large numbers of cell lines and primary cells, and it is equally suita