3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Linear polyethyleneimine (L P E I ) is a very effective transfection reagent suitable for large numbers of cell lines and primary cells, and it is equally suita
Nanoparticles combining polylactic acid-hydroxyglycolic acid (PLGA) and polyethylene glycol (PEG) derivatives have been used as gene carriers for mucosal permea
A method for efficient delivery of pol III-driven SHRNA expression frames using the Sleeping Beauty (SB) transposon. Advantages of this system include its ease
Among the various viral and non-viral means commonly used for nucleic acid transport and gene expression in animal cells, the naked plasmid D N A technique has
Introduction by microinjection can be used for any type of adherent cell, including primary cells. Since siRNA for any target gene can be obtained simply and ra
The RheoSwitch system is an efficient gene regulatory system based on the ecdysone receptor, which is activated by synthetic small molecule ligands. The recepto
Successful gene transfer requires efficient transport and long-term presence of exogenous genes in order to maintain physiologically relevant and appropriately
Transferring genes into cells and expressing new proteins, or in this way altering the expression levels of endogenous proteins, can modulate the physiological
Site-specific knockout and non-toxic DNA delivery technologies are particularly important in the study of protein function and as a means for future therapies.
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