Laboratory Protocols & SOPs Library
- Analytical experiments on the mechanism of action of unknown antihypertensive drugs SummaryGanglion blockers affect the blood pressure drop induced by direct stimulation of the vagus nerve and the boosting effect of clamping the common carotid artery, but do not affect the blood pressure changes induced by acetylcholine and adrenaline, while alpha-blockers reverse only the ...
- Experiments on the effect of different routes of administration on drug action SummaryIn pharmacology experiments, there are many factors affecting the experimental results, some of which can be controlled, while some are difficult to control. In order to reduce the influence of multi-factors and difficult-to-control factors, a control group should be set up at the same ...
- Experiments on the effect of dose on drug action SummaryThe purpose of this experiment was to observe the differences in the effects of different doses of sodium pentobarbital on mice. After the administration of the drug, the activity of the mice was observed and compared with the disappearance and recovery time of the turning reflex, so as ...
- Experimental determination of LD50 SummaryThe lethal dose (LD50) is an indication of the level of toxicity of drugs, poisons and pathogenic microorganisms. It represents the dose or concentration that kills half of all subjects. Because of the individual variability of organisms, the LD50 needs to be measured with a considerable ...
- Experimental effects of drugs on blood pressure and heart rhythm in rabbits SummaryEpinephrine alone has a pressor effect, while alpha-blockers can reverse its pressor effect. Barium chloride can promote the inward flow of sodium ions in the Purkinje fibers of the heart, inhibit the outward flow of potassium ions, promote the 4-phase automatic depolarization, and ...
- Experimental methods for the determination of blood concentration and calculation of pharmacokinetic parameters of sulfonamides SummarySulfonamides and compounds with free amino groups on the benzene ring undergo diazotization with sodium nitrite in acidic solutions to produce diazonium salts, in addition to coupling reactions with phenolic substances (e.g., muscimol) in alkaline solutions to produce reddish-orange azo ...
- Observation experiment on the diuretic effect of drugs SummaryNA is an a receptor agonist, a larger dose can constrict renal blood vessels, reduce renal blood flow, resulting in a decrease in urine output; furosemide is a highly effective diuretic, through the inhibition of renal tubular medullary collaterals of the ascending branch of the thick ...
- Experimental determination of plasma half-life and apparent volume of distribution of drugs SummaryIn pharmacology experiments, there are many factors affecting the experimental results, some of which can be controlled, while some are difficult to control. In order to reduce the influence of multi-factors and difficult-to-control factors, a control group should be set up at the same ...
- Experiments on the observation of the inhibitory and excitatory effects of drugs on respiration SummaryHigh-dose injections of aminoglycoside antibiotics can produce a blockade of the muscle junction, which is manifested by weakness of the extremities. Dyspnea or even respiratory arrest. Calcium or neostigmine may antagonize this toxic effect. Pentobarbital sodium blood concentration ...