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Denature the protein

Topic: Denature the protein

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After adding buffer to boil denatured proteins for cryopreservation, do they still need to be boiled after they are removed again? Why?
posted this at Jun 9, 2025

1
After adding buffer to boil denatured proteins for cryopreservation, do they still need to be boiled after they are removed again? Why?
What is the reason why the protein after denaturation by adding buffer tends to be difficult to sink and float up easily when spotting? What should I do to solve this problem?
posted this at Jun 9, 2025

3
What is the reason why the protein after denaturation by adding buffer tends to be difficult to sink and float up easily when spotting? What should I do to solve this problem?

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