Morphological Observation Experiment of White Lacewing
Morphological Observation Experiment of White Lacewing
This experiment is from the official website of Human Parasitology of Shandong University
Operation method
Morphological Observation Experiment of White Lacewing
Principle
White lacewing is a small blood-sucking insect, yellowish brown body, humpbacked, covered with hair, jumping flight, life history of metamorphosis, divided into eggs, larvae, pupae and adults, white lacewing species are many, the vector of black fever is the Chinese white lacewing. The main points are the pharyngeal armor, the fertilization capsule and the external genitalia of the male lacewing, and other morphological features related to classification. Move I. Observe for yourself: For more product details, please visit Aladdin Scientific website.
Chinese white lacewing (Phlebotomus chinensis) adult, pharyngeal carapace, spermatheca slide specimen: the hairs on the 2nd-6th segments of the ventral dorsum are erect, the oral carapace is undeveloped, and the anterior and middle parts of the pharyngeal carapace are composed of "V"-shaped denticles, with intermittently connected transverse ridges at the base, the female lacewing spermatheca is divided into segments (13-14 segments). The spermatophore of the female lacewing is corncob-like and segmented (13-14 segments); the male lacewing has 5 giant hairs on the second segment of the claspers above the external genitalia.
II. Demonstration:
1. General morphologic features of lacewing life history stages.
(1) Adult slide specimen: about 3 millimeters long, covered with tufts of fine hairs, the body is divided into three parts: head, thorax and abdomen.
Head: a pair of compound eyes, a pair of antennae, stinging sucking mouthparts, shorter, antennae longer than the mouthparts, divided into five segments, curved upward and backward since the fourth segment, antennae longer, mouth armor in the oral cavity, pharyngeal armor in the pharynx.
Thorax: upwardly elevated, resembling a camel's back; the mesothorax is the most developed; there is a pair of wings, which are narrow and hairy, with pointed tips; when the lacewing stops, the two wings are erected upward and at an angle of 45℃ with the body. Distribution of wing veins: the 1st, 3rd and 6th longitudinal veins are not forked, the 2nd, 4th and 5th longitudinal veins are forked, and the 2nd longitudinal vein is divided into 3 branches. The 4th and 5th longitudinal veins are each divided into 2 branches. There are 3 pairs of legs, thin and long.
Abdomen: 10 segments, segments 2-6 with slender hairs on the dorsal surface, either vertical or horizontal, or a mixture of both, depending on the species, usually vertical hairs are disease-transmitting lacewing species. The male lacewing has male external genitalia at the end of the abdomen, including a pair of upper pincers (each upper pincer is divided into upper and lower sections), the upper section of the upper pincer has giant hairs, the number and distribution of which is one of the bases for classification. There are a pair of lower pincers, a pair of intermediate appendages, a pair of penises, and a pair of caudal whiskers and reproductive filaments in the submedius. The female lacewing has a pair of spermathecae in the abdomen, the shape of which is one of the bases for classification. 
(2) Egg slide specimen: long oval, with longitudinal and transverse reticulated pattern on the shell, dark brown.
(3) Larval slide specimen: four stages, the body is divided into head, thorax and abdomen, without eyes, mouthparts are chewing. The larvae of the first stage have a pair of long setae on the tail, and the larvae of the 2nd-4th stages have two pairs of long setae on the tail.
(4) Pupal slide specimen: the fourth instar larvae shed their skin at the tail end of the pupa after maturity, and the four caudal setae on the skin of the larvae were still present.
(2) Phlebtomus mongolensis: slide specimen of spermatophore, pharyngeal armor and male external genitalia: note that it is comparable to the Chinese lacewing. Phlebtomus mongolensis has erect hairs on the back of abdominal segments 2-6. Although the hairs are erect, they do not transmit disease because of the formation of a peritrophic membrane in the stomach after sucking blood. There is no mouth armor, and the pharyngeal armor has a gravelly pattern with no transverse stripe at the end. The female lacewing's receptacle is short and divided into four segments; the upper pincer of the male lacewing's external genitalia is short and thick, with four giant hairs.