NKG2A/C/E Rat mAb (Biotin) - Primary antibody, specific to NKG2A + NKG2E + NKG2C, Rat IgG2a

Cat. No.: Ab191683

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

20D5

Conjugation

Biotin

Overview

Rat anti Mouse NKG2A/C/E Antibody, Monoclonal (20D5), could be used for Flow, IHC, in vivo NKG2A blockade, in vitro NKG2A blockade and so on.

Specifications

Product Name

NKG2A/C/E Rat mAb (Biotin) - Primary antibody, specific to NKG2A + NKG2E + NKG2C, Rat IgG2a

Synonyms

NKG2A | NKG2B | CD159a | NKG2C | Klrc2 | Nkg2e | CD159c | CD159e | Biotin Rat Anti-Mouse NKG2A, C, E | NKG2A/C/E Monoclonal Antibody (20d5), Biotin

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Rat

Specificity

NKG2A + NKG2E + NKG2C

Immunogen

CHO transfected cells expressing the C57BL/6 allele of NKG2A and CD94

Positive Control

Flow: C57BL/6 mouse splenocytes.

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Rat IgG2a

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: NKG2A/C/E Rat mAb (Biotin) (Ab191683)

NKG2A/C/E Rat mAb (Biotin) (Ab191683) - Flow Cytometry
Two-color flow cytometric analysis of NKG2A/C/E expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD19 Rat mAb (APC) (Ab185395) and either Rat IgG (Biotin) (Ab180310; Left panel) or NKG2A/C/E Rat mAb (Biotin) (Ab191683; Right panel) at 0.1 μg/test. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0 μg/test, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume ;

Associated Targets(Human)

KLRC1 Tbio NKG2-A/NKG2-B type II integral membrane protein (0 Activities)

Discover Target: KLRC1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

KLRC3 Tbio NKG2-E type II integral membrane protein (0 Activities)

Discover Target: KLRC3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

KLRC2 Tbio NKG2-C type II integral membrane protein (0 Activities)

Discover Target: KLRC2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action