RANKL/CD254 Rat mAb - Primary antibody, specific to RANKL/CD254, Rat IgG2a

Cat. No.: Ab124551

GRADE & PURITY Carrier Free, Validated, Low Endotoxin, Azide Free, PBS Only, ≥95%(SDS-PAGE&HPLC), See COA

Application

Blocking, Flow Cytometry, Functional Assay, IF/ICC, IHC, IP, IP-MS, WB

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

IK22/5

Conjugation

Unconjugated

Overview

Rat anti Mouse RANKL/CD254 Antibody, Monoclonal (IK22/5), could be used for Flow, IP, WB, IF, ICC, IHC, Blocking and so on.
Application
Flow: ≤ 0.25µg/100w cells
IP: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
Blocking: Use at an assay dependent concentration.
Protein Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Specifications

Product Name

RANKL/CD254 Rat mAb - Primary antibody, specific to RANKL/CD254, Rat IgG2a

Synonyms

Ly109l | Trance | CD254 antigen | CD254 | ODF | OPGL | OPGLOPTB2 | Osteoclast differentiation factor | Osteoprotegerin ligand | RANK L | RANKL | RANKLreceptor activator of nuclear factor kappa B ligand | Receptor activator of nuclear factor kappa-B ligand

Specifications & Purity

Carrier Free, Low Endotoxin, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE&HPLC), See COA

Host species

Rat

Specificity

RANKL/CD254

Immunogen

Purified Recombinant Mouse RANKL (NSO-derived)

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, Low Endotoxin, PBS Only, Validated

Isotype

Rat IgG2a

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

ELISA: RANKL/CD254 Rat mAb (Ab124551)

RANKL/CD254 Rat mAb (Ab124551) - ELISA
Immobilized Recombinant Mouse TRANCE/RANKL/TNFSF11 protein (rp154577) at 1.0 μg/mL can bind RANKL/CD254 Rat mAb (Ab124551) with the EC50 of 0.977 ng/mL.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.25μg/100w cells ;

• WB:

  Use at an assay dependent concentration.;

• Blocking:

  Use at an assay dependent concentration.;

• IHC:

  Use at an assay dependent concentration.;

• IP-MS:

  Use at an assay dependent concentration.;

• IF/ICC:

  Use at an assay dependent concentration.;

Associated Targets(Human)

TNFSF11 Tclin Tumor necrosis factor ligand superfamily member 11 (0 Activities)

Discover Target: TNFSF11

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action