Recombinant CD16/CD32 Antibody (AF405) - Primary antibody, specific to CD16/32

Cat. No.: Ab213309
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Ex:401nm ? Fluorescence excitation maximum at 401 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:422nm ? Fluorescence emission maximum at 422 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 0.5 mg/mL
Application
Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG2b
Clone Number
P05C29
Conjugation
AF405
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab213309-10μg
3
$71.90
25μg
Ab213309-25μg
1
$143.90
100μg
Ab213309-100μg
1
$355.90
Enter a quantity for the sizes you want to add.
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Why this grade

ExactAb™, Validated, Ex:401nm, Em:422nm, 0.5 mg/mL Em:422nm,Ex:401nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Recombinant CD16/CD32 Antibody, clone P05C29, could be used for Flow Cytometry and so on.

Specifications

Product Name
Recombinant CD16/CD32 Antibody (AF405) - Primary antibody, specific to CD16/32
Synonyms
CD32 | Fcgr2 | Fcr-2 | Fcr-3 | Ly-17 | LyM-1 | fcRII | FcgRII | Fcgr2a | Ly-m20 | Fc[g]RII | F630109E10Rik | CD16 | CD16A | CD16-II | FCG3 | FCGR3 | FCGR3A | FCGRIII | FcGRIIIA | FCR-10 | FCRIII | FCRIIIA | IGFR3 | IMD20 | Low affinity immunoglobulin gamm
Specifications & Purity
ExactAb™, Validated, Ex:401nm, Em:422nm, 0.5 mg/mL
Biochemical and Physiological Mechanisms
CD16 and CD32 serve as low affinity receptors for IgG Fc constant regions and are involved in regulating various cellular functions including antibody-dependent cellular toxicity (ADCC), phagocytosis, effector cell degranulation, and B cell proliferation.
Host species
Rat
Specificity
CD16/32
Positive Control
Flow: C57BL/6 mouse splenocytes
Conjugation
AF405
Excitation (nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
Grade
Em:422nm, Ex:401nm, ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

Recombinant CD16/CD32 Antibody (AF405) (Ab213309) - Flow Cytometry
Two-color flow cytometric analysis of CD16/CD32 expression on C57BL/6 Mouse splenocytes. C57BL/6 Mouse splenocytes cells were stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG (AF405) (Ab190070; Left panel) or Recombinant CD16/CD32 Antibody (AF405) (Ab213309; Right panel) at 0.2μg/test.

Application
ApplicationDilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
FCGR2B Tbio Low affinity immunoglobulin gamma Fc region receptor II-b (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F1216278Certificate of AnalysisJul 10, 2026 Ab213309
ZJ24F1216279Certificate of AnalysisJul 10, 2026 Ab213309
ZJ24F1216280Certificate of AnalysisJul 10, 2026 Ab213309
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Ex:401nm grade guide → View Em:422nm grade guide →

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