Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti His tag Antibody, Recombinant, could be used for WB, IF/ICC, Flow Cytometry, IP and so on.
Recombinant His tag Antibody (Ab086830) - Western Blot
All lanes: Recombinant His tag Antibody (Ab086830) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 116 kDa
Observed band size: 47, 55,112 kDa
Exposure time: 0.3 s
Recombinant His tag Antibody(Ab086830) - IF/ICC
Recombinant His tag Antibody (Ab086830) staining HEK293 cells trasnfected with N-terminal 6X His tagged gene by immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant His tag Antibody (Ab086830) (1/10000) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An AF488-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant His tag Antibody(Ab086830) - IF/ICC
Recombinant His tag Antibody (Ab086830) staining HEK293 cells trasnfected with C-terminal 6X His tagged gene by immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant His tag Antibody (Ab086830) (1/800) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An AF488-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant His tag Antibody(Ab086830) - IP
His tag was immunoprecipitated from 0.05 mg of HEK293 whole cells lysate transfected with N-terminal 6X His tagged gene with Recombinant His tag Antibody (Ab086830) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant His tag Antibody(Ab086830) IP in HEK293 whole cell lysate transfected with N-terminal 6X His tagged gene
Lane 2: PBS instead of Recombinant His tag Antibody(Ab086830) in HEK293 whole cell lysate transfected with N-terminal 6X His tagged gene
Lane 3: HEK293 whole cell lysate transfected with N-terminal 6X His tagged gene, 10 μg (input)
Exposure: 20.0s
Recombinant His tag Antibody(Ab086830) - IP
His tag was immunoprecipitated from 0.2 mg of HEK293 whole cells lysate transfected with C-terminal 6X His tagged gene with Recombinant His tag Antibody(Ab086830) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant His tag Antibody(Ab086830) IP in HEK293 whole cells lysate transfected with C-terminal 6X His tagged gene
Lane 2: PBS instead of Recombinant His tag Antibody(Ab086830) in HEK293 whole cells lysate transfected with C-terminal 6X His tagged gene
Lane 3: HEK293 whole cells lysate transfected with C-terminal 6X His tagged gene, 10 μg (input)
Exposure: 120.0s
Recombinant His tag Antibody(Ab086830) - Flow Cytometry
Overlay histogram showing HEK293 cells transfected with N-terminal (Blue) and C-terminal (Red) 6X His tagged gene stained with Recombinant His tag Antibody (Ab086830). The cells were then incubated in the antibody (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
| Application | Dilution info |
|---|---|
| WB | 1/1000 - 1/2000 |
| IF/ICC | 1/800 - 1/2000 |
| IP |
1/50
|
| Flow Cytometry |
1/200 - 1/1000
|
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Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0506203 | Certificate of Analysis | Mar 19, 2026 | Ab086830 |
| ZJ24F0506204 | Certificate of Analysis | Mar 19, 2026 | Ab086830 |
| ZJ24F0506205 | Certificate of Analysis | Mar 19, 2026 | Ab086830 |
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