Recombinant Hsp90 Antibody - Primary antibody, specific to HSP90AA1, Rabbit IgG

Cat. No.: Ab108929

GRADE & PURITY Recombinant, Validated, ExactAb™, See COA

Application

IF/ICC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human HSP90 Antibody, Recombinant, could be used for WB, IF/ICC and so on.

Specifications

Product Name

Recombinant Hsp90 Antibody - Primary antibody, specific to HSP90AA1, Rabbit IgG

Synonyms

FLJ31884 | Heat shock 86 kDa | heat shock 90kD protein 1 | heat shock 90kD protein | heat shock 90kDa protein 1 | heat shock protein 90kDa alpha (cytosolic) | heat shock protein HSP 90-alpha | Hsp89 | HSP90 | HSP90AHSP86 | HSP90N | HSPC1HSP 86 | HSPCAHSP8

Specifications & Purity

Recombinant, ExactAb™, Validated, See COA

Biochemical and Physiological Mechanisms

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activ

Host species

Rabbit

Specificity

HSP90AA1

Immunogen

A synthetic peptide derived from human Hsp90 alpha (AA 1-52).

Positive Control

WB: Jurkat, Hep G2, K562, LNCaP, MCF7, A549, HeLa, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant Hsp90 Antibody (Ab108929)

Recombinant Hsp90 Antibody (Ab108929) - Western Blot
All lanes: Recombinant Hsp90 Antibody (Ab108929) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 85 kDa
Observed band size: 89 kDa
Exposure time: 13.5 s

IF/ICC: Recombinant Hsp90 Antibody (Ab108929)

Recombinant Hsp90 Antibody (Ab108929) - IF/ICC
Immunofluorescence analysis of Hsp90 alpha in HeLa using Recombinant Hsp90 Antibody (Ab108929), and cells were counterstained with DAPI (blue).
.

Application

• Application: Dilution info
• IF/ICC:

  1/50-1/200 ;

• WB:

  1/1000 - 1/2000

Associated Targets(Human)

HSP90AA1 Tchem Heat shock protein HSP 90-alpha (0 Activities)

Discover Target: HSP90AA1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HSP90AA1 Tchem Heat shock protein HSP 90-alpha (4115 Activities)

Discover Target: HSP90AA1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

HSP90AA1 Tchem Heat shock protein HSP90 (3606 Activities)

Discover Target: HSP90AA1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

References

1. Qun Wei, Jun-Ya Ning, Xi Dai, Yuan-Di Gao, Le Su, Bao-Xiang Zhao, Jun-Ying Miao.  (2018)  Discovery of novel HSP90 inhibitors that induced apoptosis and impaired autophagic flux in A549 lung cancer cells.  EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY,      [PMID:29339250] [10.1016/j.ejmech.2018.01.024]

2. Yuanyuan Wang, Hao Ma, Jiaxuan Huang, Zhengguang Yao, Jianqiang Yu, Wannian Zhang, Lichao Zhang, Zhibin Wang, Chunlin Zhuang.  (2020)  Discovery of bardoxolone derivatives as novel orally active necroptosis inhibitors.  EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY,      [PMID:33248849] [10.1016/j.ejmech.2020.113030]