Recombinant Human CD40 Ligand/TNFSF5 Protein, >98% SDS-PAGE

Cat. No.: rp169583

GRADE & PURITY Carrier Free, Bioactive, ActiBioPure™, Azide Free, His Tag, ≥98%(SDS-PAGE)

Expression System

E. coli

Accession #

P29965

Protein Tag

N-His

Expression system

E. coli

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Testing in progress

Overview

Purity:>98%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description：
CD40 Ligand, also known as TNFSF, CD154, TRAP, and gp39, is a 34-39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily. Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region. The extracellular domain of human CD40 Ligand shares 74% and 76% aa sequence identity with mouse and rat CD40 Ligand, respectively. Similar to other TNF superfamily members, CD40 Ligand forms a bioactive homotrimer, both as membrane bound and soluble forms. The 18 kDa soluble form (aa 113-261) arises from proteolytic processing. Mutation and alternative splicing generate additional forms of CD40 Ligand that are often truncated or non-trimerizable. CD40 Ligand is expressed on platelets, as well as on activated T cells and B cells, basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds to CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial, and epithelial cells. The interaction of CD40 Ligand with CD40 initiates signaling in both CD40 and CD40 Ligand expressing cells. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.

Specifications

Product Name

Recombinant Human CD40 Ligand/TNFSF5 Protein, >98% SDS-PAGE

Synonyms

CD154 antigen | CD154 | CD40 antigen ligand | CD40 Ligand | CD40L | CD40-L | CD40LG | CD40LIGM | gp39 | hCD40L | HIGM1 | T-B cell-activating molecule | T-BAM | T-cell antigen Gp39 | TNF-related activation protein | TNFSF5 | TNFSF5IMD3 | TRAP | TRAPtumor n

Grade

ActiBioPure™, Azide Free, Bioactive, Carrier Free, His Tag

Specifications & Purity

Carrier Free, Bioactive, ActiBioPure™, Azide Free, His-Tag, ≥98%(SDS-PAGE)

Purity

>98% SDS-PAGE

Bioactivity

Testing in progress

Endotoxin Concentration

<1.0 EU/μg

Expression System

E. coli

Amino Acids

108-261 aa

Sequence

MHHHHHHENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

Protein Tag

N-His

Accession #

P29965

Predicted molecular weight

17.7 kDa

SDS-PAGE

16.0 kDa, reducing conditions; 16.0 kDa, non-reducing conditions

Molecule Type

Protein

Storage and Shipping

Shape

Transparent gelatinous

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C stable up to 1 year. Avoid freeze / thaw cycle.

Images

SDS-PAGE: Recombinant Human CD40 Ligand/TNFSF5 Protein (rp169583)

Recombinant Human CD40 Ligand/TNFSF5 Protein (rp169583) - SDS-PAGE
Recombinant Human CD40 Ligand/TNFSF5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16.0 kDa.