GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
P00533
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Depatuxizumab (anti-EGFR) (Ab182750) with the EC50 of 25.83 ng/mL. Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Depatuxizumab mafodotin (anti-EGFR) (Ab175508) with the EC50 of 28.96 ng/mL. Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Nimotuzumab (anti-EGFR) (Ab175612) with the EC50 of 45.18 ng/mL. Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Serclutamab (anti-EGFR) (Ab182867) with the EC50 of 35.7 ng/mL. Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Cetuximab (anti-EGFR) (C411992) with the EC50 of 17.44 ng/mL. Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Panitumumab (anti-EGFR) (Ab169317) with the EC50 of 23.66 ng/mL.
Animal Free,Carrier Free,Bioactive,ActiBioPure™,His Tag,PBS Only,≥95%(SDS-PAGE) ActiBioPure™,Animal Free,Bioactive,Carrier Free,His Tag,PBS Only for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Protein Function： As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Specifications
Product Name
Recombinant Human EGFR Protein
Synonyms
cell growth inhibiting protein 40 | avian erythroblastic leukemia viral (v-erb-b) oncogene homolog | cell proliferation-inducing protein 61 | EC 2.7.10 | EC 2.7.10.1 | EGF R | EGFR | epidermal growth factor receptor (avian erythroblastic leukemia viral (v
Grade
ActiBioPure™, Animal Free, Bioactive, Carrier Free, His Tag, PBS Only
Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Depatuxizumab (anti-EGFR) (Ab182750) with the EC50 of 25.83 ng/mL. Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Depatuxizumab mafodotin (anti-EGFR) (Ab175508) with the EC50 of 28.96 ng/mL. Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Nimotuzumab (anti-EGFR) (Ab175612) with the EC50 of 45.18 ng/mL. Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Serclutamab (anti-EGFR) (Ab182867) with the EC50 of 35.7 ng/mL. Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Cetuximab (anti-EGFR) (C411992) with the EC50 of 17.44 ng/mL. Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Panitumumab (anti-EGFR) (Ab169317) with the EC50 of 23.66 ng/mL.
99.6 kDa, under reducing conditions; 99.6 kDa, under non-reducing conditions
Molecule Type
Protein
Storage and Shipping
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C for 1 year. Avoid freeze / thaw cycle.
Images
Recombinant Human EGFR Protein (rp176273) - ELISA Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Depatuxizumab (anti-EGFR) (Ab182750) with the EC₅₀ of 25.83 ng/mL.
Recombinant Human EGFR Protein (rp176273) - ELISA Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Depatuxizumab mafodotin (anti-EGFR) (Ab175508) with the EC50 of 28.96 ng/mL.
Recombinant Human EGFR Protein (rp176273) - ELISA Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Nimotuzumab (anti-EGFR) (Ab175612) with the EC₅₀ of 45.18 ng/mL.
Recombinant Human EGFR Protein (rp176273) - ELISA Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Serclutamab (anti-EGFR) (Ab182867) with the EC₅₀ of 35.7 ng/mL.
Recombinant Human EGFR Protein (rp176273) - ELISA Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Cetuximab (anti-EGFR) (C411992) with the EC₅₀ of 17.44 ng/mL.
Recombinant Human EGFR Protein (rp176273) - ELISA Immobilized Recombinant Human EGFR Protein (rp176273) at 1.0 μg/mL can bind Panitumumab (anti-EGFR) (Ab169317) with the EC₅₀ of 23.66 ng/mL.
Recombinant Human EGFR Protein (rp176273) - SDS-PAGE 2 μg/lane of Recombinant Human EGFR Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 99.6 kDa.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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