Recombinant Human HTRA1 Protein

Cat. No.: rp179843

GRADE & PURITY Carrier Free, Bioactive, ActiBioPure™, His Tag, ≥90%(SDS-PAGE)

Expression System

E. coli

Accession #

Q92743

Protein Tag

C-His

Expression system

E. coli

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Casein (0.5 mg/mL) was incubated in 50mM Tris, pH 8.0, or with Recombinant Human HTRA1 Protein (rp179843). After various time intervals aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.

Overview

Protein Function:：
HTRA1 is a member of the mammalian HTRA (high temperature requirement A) serine protease family. The E. coli HTRA homolog functions as a chaperone-protease and is essential for bacterial survival at temperatures above 42 °C. Among the four mammalian HTRA proteins, HTRA1, -3 and -4 are secreted while HTRA2 is localized in mitochondria. HTRA1 contains an N-terminal insulin-like growth factor binding protein (IGFBP) domain, a Kazal-type trypsin inhibitor motif, and C-terminal trypsin-like protease and PDZ domains. It is involved in several pathologies including Alzheimer’s disease, rheumatoid arthritis, osteoarthritis and age‑related macular degeneration, although the mechanisms by which HTRA1 exerts its effects are not clear. HTRA1 also has properties of a tumor suppressor protein, which makes it a target for cancer therapy. In addition HTRA1 is known to regulate the TGF-beta signaling pathway and bone mineralization. Recombinant human HTRA1 lacks the N-terminal IGFBP and Kazal-like domains, but retains the serine protease and PDZ domains.

Specifications

Product Name

Recombinant Human HTRA1 Protein

Synonyms

EC 3.4.21 | ARMD7 | EC 3.4.21.108 | High-temperature requirement A serine peptidase 1 | HtrA serine peptidase 1 | HTRA1 | IGFBP5-protease | L56 | ORF480 | PRSS11 | Serine protease 11 | serine, 11 (IGF binding)

Grade

ActiBioPure™, Bioactive, Carrier Free, His Tag

Specifications & Purity

Carrier Free, Bioactive, ActiBioPure™, His-Tag, ≥90%(SDS-PAGE)

Bioactivity

Casein (0.5 mg/mL) was incubated in 50mM Tris, pH 8.0, or with Recombinant Human HTRA1 Protein (rp179843). After various time intervals aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.

Endotoxin Concentration

<1.0 EU/μg

Expression System

E. coli

Amino Acids

156-480 aa

Sequence

MGQGQEDPNSLRHKYNFIADVVEKIAPAVVHIELFRKLPFSKREVPVASGSGFIVSEDGLIVTNAHVVTNKHRVKVELKNGATYEAKIKDVDEKADIALIKIDHQGKLPVLLLGRSSELRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDKIKKFLTESHDRQAKGKAITKKKYIGIRMMSLTSSKAKELKDRHRDFPDVISGAYIIEVIPDTPAEAGGLKENDVIISINGQSVVSANDVSDVIKRESTLNMVVRRGNEDIMITVIPEEIDPHHHHHH

Protein Tag

C-His

Accession #

Q92743

Predicted molecular weight

36.3 kDa

SDS-PAGE

35.6 kDa, under reducing conditions; 35.6 kDa, under non-reducing conditions

Molecule Type

Protein

Storage and Shipping

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

Western Blot: Recombinant Human HTRA1 Protein (rp179843)

Recombinant Human HTRA1 Protein (rp179843) - Western Blot
0.5 mg/mL Casein(C110500) was incubated in 50mM Tris, pH 8.0, or with Recombinant Human HTRA1 Protein (rp179843). After various time intervals aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.

SDS-PAGE: Recombinant Human HTRA1 Protein (rp179843)

Recombinant Human HTRA1 Protein (rp179843) - SDS-PAGE
3 μg/lane of Recombinant Human HTRA1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 35.6 kDa under reducing conditions and 35.6 kDa under non-reducing conditions.