GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Protein Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Specifications
Product Name
Recombinant NDUFB4 Antibody - Primary antibody, specific to NDUFB4, Rabbit IgG
WB: Mouse skeletal muscle, K562, Hep G2, NIH/3T3 and C6 lysates. IHC: Human colon cancer tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant NDUFB4 Antibody (Ab117371) - Western Blot All lanes: Recombinant NDUFB4 Antibody (Ab117371) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 20.0 s
Recombinant NDUFB4 Antibody (Ab117371) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant NDUFB4 Antibody (Ab117371). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant NDUFB4 Antibody (Ab117371) - IF/ICC Immunocytochemistry analysis of NDUFB4 (green) in HeLa using Recombinant NDUFB4 Antibody (Ab117371), and cells were counterstained with DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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