GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Recombinant, ExactAb™, Validated, High Performance, See COA ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human NSE Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on
Specifications
Product Name
Recombinant NSE Antibody - Primary antibody, specific to NSE
Recombinant, ExactAb™, Validated, High Performance, See COA
Biochemical and Physiological Mechanisms
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Host species
Rabbit
Specificity
NSE
Immunogen
A synthetic peptide derived from human gamma Enolase (AA 1-60)
Positive Control
WB: HeLa, Jurkat, Hep G2, MCF7, SH-SY5Y, NIH/3T3 and C6 cell lysates. IHC: Human uterus cancer tissue. IF/ICC: A431 cells.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant NSE Antibody (Ab118791) - Western Blot All lanes: Recombinant NSE Antibody (Ab118791) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1.8 s
Recombinant NSE Antibody (Ab118791) - IHC Immunohistochemical analysis of paraffin-embedded human uterus cancer, using Recombinant NSE Antibody (Ab118791).
Recombinant NSE Antibody (Ab118791) - IF/ICC Immunofluorescent analysis of A431 cells, using Recombinant NSE Antibody (Ab118791).
Application
Application
Dilution info
IHC
1/100 - 1/200
IF/ICC
1/50 - 1/200
WB
1/1000-1/5000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
1.Xu Kun, Dai Li, Du Yu, Liu Lei, Zhang Nuo, Feng Ruiqing, Xu Rui, Wei Qin. (2024) A signal polarity conversion photoelectrochemical immunosensor for neuron-specific enolase detection based on MgIn2S4-sensitized CsPbBr3. MICROCHIMICA ACTA, 191 (2):(1-8). [PMID:38195951][10.1007/s00604-023-06174-3]
Solution Calculators
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Reconstitution Calculator
Reviews
Customer Reviews
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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