7-Aminoactinomycin D - Moligand™, ≥97%(HPLC) , CAS No.7240-37-1

CAS: 7240-37-1 Cat. No.: A131295 Molecular Weight: 1270.43 EC Number: 635-285-6

GRADE & PURITY Moligand™, ≥97%(HPLC)

Synonyms

7-amino-AMD | CHEBI:52304 | UNII-DXS32PH95R | Actinomycin D, 7-Amino- - CAS 7240-37-1 | PD165780 | Actinomycin D, 7-amino- | MFCD00077295 | 7-Aminoaktinomycin D | 7-AAD | Q259277 | 2,7-diamino-4,6-dimethyl-3-oxo-1-N,9-N-bis-[(18aS)-10c,14,17-trimethyl-5,8

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

7-Aminoactinomycin D ，an analog of the antibiotic actinomycin D ，is a fluorescent DNA dye which is useful for quantifying apoptosis. 7-AAD has also been used in studies on cellular necrosis, chromosome analysis and in cell cycle studies. As a fluorescent DNA dye, 7-AAD is able to intercalate DNA via binding to the GC regions. When used in flow cytometery, 7-AAD can be used to discriminate between live cells as well as early and late apoptosis. Additionally, 7-AAD can also be combined with other staining procedures for analysis of intracellular and cell surface antigens enabling multiparametric analysis. When utilizing 7-AAD with flow cytometery, early and late apoptosis can be studied by varying the concentration of 7-AAD. During apoptosis, the cellular plasma membrane is altered progressively, which allows the cell membrane to become permeable to 7-AAD. 7-AAD staining can also be adapted to include staining with other dyes for intracellular and cell surface antigens. 7-Aminoactinomycin D is an inhibitor of DNA Polymerase and RNA Polymerase
A fluorescent DNA dye for apoptosis and flow cytomtery studies.

Specifications

Synonyms

7-amino-AMD | CHEBI:52304 | UNII-DXS32PH95R | Actinomycin D, 7-Amino- - CAS 7240-37-1 | PD165780 | Actinomycin D, 7-amino- | MFCD00077295 | 7-Aminoaktinomycin D | 7-AAD | Q259277 | 2, 7-diamino-4, 6-dimethyl-3-oxo-1-N, 9-N-bis-[(18aS)-10c, 14, 17-trimethyl-5, 8

Specifications & Purity

Moligand™, ≥97%(HPLC)

Biochemical and Physiological Mechanisms

Fluorescent DNA dye for apoptosis and flow cytometry studies. Guanine-specific synthetic fluorescent analogue of actinomycin D. Intercalates DNA by binding to GC regions. Discriminates between live cells as well as early and late apoptosis in flow cytomet

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Action Type

INHIBITOR

Note

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Purity

≥97%(HPLC)

Names and Identifiers

• Pubchem Sid: 504767737
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504767737
• Canonical Smiles: CC1C(C(=O)NC(C(=O)N2CCCC2C(=O)N(CC(=O)N(C(C(=O)O1)C(C)C)C)C)C(C)C)NC(=O)C3=CC(=C(C4=C3N=C5C(=C(C(=O)C(=C5O4)C)N)C(=O)NC6C(OC(=O)C(N(C(=O)CN(C(=O)C7CCCN7C(=O)C(NC6=O)C(C)C)C)C)C(C)C)C)C)N
• IUPAC Name: 2,7-diamino-4,6-dimethyl-3-oxo-1-N,9-N-bis[(3R,6S,7R,10S,16S)-7,11,14-trimethyl-2,5,9,12,15-pentaoxo-3,10-di(propan-2-yl)-8-oxa-1,4,11,14-tetrazabicyclo[14.3.0]nonadecan-6-yl]phenoxazine-1,9-dicarboxamide
• InChIKey: YXHLJMWYDTXDHS-IRFLANFNSA-N
• INCHI: 1S/C62H87N13O16/c1-26(2)42-59(85)74-21-17-19-36(74)57(83)70(13)24-38(76)72(15)48(28(5)6)61(87)89-32(11)44(55(81)66-42)68-53(79)34-23-35(63)30(9)51-46(34)65-47-40(41(64)50(78)31(10)52(47)91-51)54(80)69-45-33(12)90-62(88)49(29(7)8)73(16)39(77)25-71(14)58(84)37-20-18-22-75(37)60(86)43(27(3)4)67-56(45)82/h23,26-29,32-33,36-37,42-45,48-49H,17-22,24-25,63-64H2,1-16H3,(H,66,81)(H,67,82)(H,68,79)(H,69,80)/t32-,33-,36+,37+,42-,43-,44+,45+,48+,49+/m1/s1
• Isomeric SMILES: C[C@@H]1[C@@H](C(=O)N[C@@H](C(=O)N2CCC[C@H]2C(=O)N(CC(=O)N([C@H](C(=O)O1)C(C)C)C)C)C(C)C)NC(=O)C3=CC(=C(C4=C3N=C5C(=C(C(=O)C(=C5O4)C)N)C(=O)N[C@H]6[C@H](OC(=O)[C@@H](N(C(=O)CN(C(=O)[C@@H]7CCCN7C(=O)[C@H](NC6=O)C(C)C)C)C)C(C)C)C)C)N
• WGK Germany: 3
• RTECS: AU1579000
• Molecular Weight: 1270.43
• Reaxy-Rn: 36726997
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=36726997&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Peptidomimetics
      • Subclass Depsipeptides

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Peptidomimetics
• Subclass: Depsipeptides
• Intermediate Tree Nodes: Not available
• Direct Parent: Cyclic depsipeptides
• Alternative Parents: Macrolide lactams Phenoxazines Macrolactams Alpha amino acid esters N-acyl-alpha amino acids and derivatives Aminobenzamides Dicarboxylic acids and derivatives Pyrrolidines Heteroaromatic compounds Vinylogous amides Tertiary carboxylic acid amides Secondary carboxylic acid amides Lactones Lactams Cyclic ketones Carboxylic acid esters Azacyclic compounds Oxacyclic compounds Organopnictogen compounds Organic oxides Primary amines Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Cyclic depsipeptide - Macrolide lactam - Phenoxazine - Alpha-amino acid ester - N-acyl-alpha amino acid or derivatives - Macrolactam - Alpha-amino acid or derivatives - Aminobenzamide - Dicarboxylic acid or derivatives - Benzenoid - Heteroaromatic compound - Pyrrolidine - Tertiary carboxylic acid amide - Vinylogous amide - Carboxamide group - Lactam - Carboxylic acid ester - Secondary carboxylic acid amide - Amino acid or derivatives - Cyclic ketone - Lactone - Carboxylic acid derivative - Oxacycle - Azacycle - Organoheterocyclic compound - Amine - Organic oxygen compound - Organic nitrogen compound - Hydrocarbon derivative - Carbonyl group - Organic oxide - Organopnictogen compound - Primary amine - Organooxygen compound - Organonitrogen compound - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as cyclic depsipeptides. These are natural or synthetic compounds having sequences of amino and hydroxy carboxylic acid residues (usually α-amino and α-hydroxy acids) connected in a ring. The residues are commonly but not necessarily regularly alternating.
• External Descriptors: chromopeptide

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Associated Targets(Human)

TERT Tchem Telomerase reverse transcriptase (2428 Activities)

Discover Target: TERT

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

CCRF-CEM (65223 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Associated Targets(non-human)

pol Human immunodeficiency virus type 1 reverse transcriptase (18245 Activities)

Discover Target: pol

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Chemical and Physical Properties

• Solubility: Soluble in DMSO (20 mg/ml), ethanol, chloroform (1 mg/ml), and dichloromethane (20 mg/ml).
• Sensitivity: Moisture & Light sensitive
• Melt Point(°C): 252-253°C
• Molecular Weight: 1270.400 g/mol
• XLogP3: 3.100
• Hydrogen Bond Donor Count: 6
• Hydrogen Bond Acceptor Count: 19
• Rotatable Bond Count: 8
• Exact Mass: 1269.64 Da
• Monoisotopic Mass: 1269.64 Da
• Topological Polar Surface Area: 382.000 Ų
• Heavy Atom Count: 91
• Formal Charge: 0
• Complexity: 3070.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 10
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

1. Jiaqi Lu, Shuting Fang, Qiaoli Lu, Chang Liu, Baohua Huang, Changqing Yao, Yanchun Hong, Qijun Ruan, Huihui Ti.  (2026)  Lactoferrin amyloid-templated gold nanozymes combat pathogenic Bacteria via a synergistic assault of oxidative stress and metabolic collapse.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,      [PMID:] [10.1016/j.jcis.2026.140292]