Conservar a -20°C,Evitar la congelación y descongelación repetidas Ships Hielera + almohadillas de hielo Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

El anticuerpo policlonal de conejo anti AKT humana (fosfo S473) puede utilizarse para WB, IHC, IF/ICC, etc

Specifications

Product Name

Anticuerpo AKT(fosfo S473) - Primary antibody, specific to AKT (phospho S473), Rabbit IgG

Sinónimos

Especificaciones y pureza

Validado, ExactAb™, Ver COA

Especies hospedadoras

Rabbit

Especificidad

AKT (phospho S473)

Inmunógeno

A synthetic peptide derived from human AKT (phospho S473) (AA 431-480).

Control positivo

WB: HeLa, 3T3 lysates. IHC: Human breast carcinoma, Human lung, Human uterus, Mouse testis, Rat brain, Rat kidney, Rat spinal cord, Rat lung, Rat testis tissues. IF/ICC: Rat liver tissue.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150kDa

Método de purificación

Immunogen affinity purified

Concentración

See COA

Almacenamiento y envío

Forma

Líquido

Condiciones de almacenamiento de almacenamiento

Conservar a -20°C,Evitar la congelación y descongelación repetidas

Enviado en

Hielera + almohadillas de hielo

Estabilidad y almacenamiento

Almacenar a -20℃ largo plazo (12 meses). Tras la recepción, se recomienda alícuota. Evite el ciclo de congelación/descongelación.

Imágenes

Western Blot: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - Western Blot
All lanes: AKT(phospho S473) Antibody (Ab087899) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 57 kDa
Exposure time: 16.1 s

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IF/ICC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IF/ICC
Immunofluorescence analysis of rat-liver tissue.
1. AKT(phospho S473) Antibody (Ab087899) (red) was diluted at 1/200 (4°C, overnight).
2. Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
3. Picture B: DAPI (blue) 10 min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B

IF/ICC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IF/ICC
Immunofluorescence analysis of rat-liver tissue.
1. AKT(phospho S473) Antibody (Ab087899)(red) was diluted at 1/200 (4°C, overnight).
2. Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
3. Picture B: DAPI (blue) 10 min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B

IF/ICC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IF/ICC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AKT(phospho S473) Antibody (Ab087899). The picture on the right is blocked with the phospho peptide.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue.

1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Human-lung tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).

2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

Aplicación

Aplicación	Dilution info
WB	1/500-1/2000
IF/ICC	1/50 - 1/200
IHC	1/100-1/300

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)

AKT3 Tchem RAC-gamma serine/threonine-protein kinase (0 Activities)

Discover Target: AKT3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

AKT2 Tchem RAC-beta serine/threonine-protein kinase (0 Activities)

Discover Target: AKT2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

AKT1 Tchem RAC-alpha serine/threonine-protein kinase (0 Activities)

Discover Target: AKT1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Certificados (CoA, COO, BSE/TSE y tabla de análisis)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number	Certificate Type	Fecha	Articulo
ZJ24F0707776	Certificate of Analysis	Apr 17, 2026	Ab087899
ZJ24F0707777	Certificate of Analysis	Apr 17, 2026	Ab087899
ZJ25F0319278	Certificate of Analysis	Jan 20, 2026	Ab087899
ZJ25F0319279	Certificate of Analysis	Jan 20, 2026	Ab087899

Preguntas frecuentes y artículos

Protocol of Western blot for Phosphorylated Proteins

Categories: Protocols

Tags:

Western blot ECL Phosphorylated proteins Phosphorylated Antibodies Immunoblot HRP-conjugated Antibodies

Protein Dephosphorylation Protocol

Categories: Protocols

Tags:

SDS-PAGE Alkaline phosphatase Phosphorylated Antibodies Protein Electrophoresis AP CIP Dephosphorylation Calf intestinal alkaline phosphatase Transfer

Composition, Activation Mechanisms, and Biological Effects of the AKT Pathway

Categories: Technical articles

Tags:

metabolic regulation GSK3 AKT PKB PI3K PTEN PDK1 mTORC2 FOXO cell survival

Angiopoietin-TIE2 Signaling Pathway: Mechanisms of Vascular Homeostasis, Permeability Regulation, and Pathological Vascular Remodeling

Categories: Technical articles

Tags:

VEGF Angiogenesis Angiopoietin endothelial barrier Vascular permeability TIE2 TEK ANGPT1 ANGPT2 TIE1 pericytes tumor vessels inflammatory microenvironment endothelial homeostasis

Calculadoras de soluciones

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Reconstitution Calculator

Reseñas

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide →

Bulk & Custom Packaging Need larger quantities or custom specifications? Contact our team for a tailored quote.