ExactAb™, Validated, Carrier Free, 1.0mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 2 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Application:
WB: 1/500 - 1/2000
IF/ICC: 1/200 - 1/1000
Flow: 1/200 - 1/400
ELISA: 1/10000
Protein Function
Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
AKT1 Mouse mAb (Ab087937) - Western Blot
All lanes: AKT1 Mouse mAb (Ab087937) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 55.7 kDa
Observed band size: 55.1 kDa
AKT1 Mouse mAb (Ab087937) - Flow Cytometry
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling AKT1 with AKT1 Mouse mAb (Ab087937) at 1/200 dilution (green) compared with a negative control (red).
AKT1 Mouse mAb (Ab087937) - ICC/IF
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for AKT1 (green) using AKT1 Mouse mAb (Ab087937) in ICC/IF. Blue: DRAQ5 DNA dye. Red: Actin filaments stained with AF-555-Phalloidin.
| Aplicación | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| IF/ICC |
1/200-1/1000
|
| Flow Cytometry |
1/200-1/400
|
| ELISA |
1/10000
|
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 ZJ23F0700655 | Certificate of Analysis | May 19, 2026 | Ab087937 |
| ZJ23F0700656 | Certificate of Analysis | May 19, 2026 | Ab087937 |
| 1. Wenhu Zhan, Jinxin Che, Lei Xu, Yizhe Wu, Xiaobei Hu, Yubo Zhou, Gang Cheng, Yongzhou Hu, Xiaowu Dong, Jia Li. (2019) Discovery of pyrazole-thiophene derivatives as highly Potent, orally active Akt inhibitors. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, [PMID:31301565] [10.1016/j.ejmech.2019.07.017] |
| 2. Peng Zhu, Jianqiang Qian, Zhongyuan Xu, Chi Meng, Ji Liu, Wenpei Shan, Weizhong Zhu, Yongjun Wang, Yumin Yang, Wei Zhang, Yanan Zhang, Yong Ling. (2020) Piperlonguminine and Piperine Analogues as TrxR Inhibitors that Promote ROS and Autophagy and Regulate p38 and Akt/mTOR Signaling. JOURNAL OF NATURAL PRODUCTS, [PMID:33026807] [10.1021/acs.jnatprod.0c00599] |
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