CD14 Mouse mAb - Primary antibody, specific to CD14

Cat. No.: Ab093874
Disponible para pedir
CALIDAD & PUREZA ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. See COA
Application
Flow Cytometry, WB
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG1
Clone Number
OTI4H4
Conjugation
Unconjugated
★
Talla
USA
Alemania (EU)*
Precio
Cantidad
10μl
Ab093874-10μl
1 Disponible
—
99,90US$
50μl
Ab093874-50μl
1 Disponible
—
259,90US$
100μl
Ab093874-100μl
Fabricado bajo pedido en EE. UU. · 2–4 semanas ·
—
419,90US$
1ml
Ab093874-1ml
Fabricado bajo pedido en EE. UU. · 2–4 semanas ·
—
3.399,90US$
Enter a quantity for the sizes you want to add.

Descripción general

Mouse anti Human CD14 Antibody, Monoclonal (OTI4H4), could be used for WB, Flow Cytometry and so on.

Especificaciones

Product Name
CD14 Mouse mAb - Primary antibody, specific to CD14
Sinónimos
CD14 antigen|CD14 molecule|CD14|monocyte differentiation antigen CD14|Myeloid cell-specific leucine-rich glycoprotein|CD 14 antibody|CD_antigen=CD14 antibody|CD14 antibody|CD14 antigen antibody|CD14 molecule antibody|LPS-R antibody|Mo2 antibody|Monocyte differentiation antigen CD14 antibody|Monocyte differentiation antigen CD14 urinary form antibody|Monocyte differentiation antigen CD14, membrane-bound form antibody|Myeloid cell specific leucine rich glycoprotein antibody|Myeloid cell-specific leucine-rich glycoprotein antibody
Especificaciones y pureza
ExactAb™, Validated, See COA
Mecanismos bioquímicos y fisiológicos
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molec
Especies hospedadoras
Mouse
Especificidad
CD14
Inmunógeno
Recombinant protein of human CD14 (AA 20-367)
Control positivo
WB: HL60 and A549 cell lysates. Flow Cytometry: HEK293T cells transfected with pCMV6-ENTRY CD14.
Conjugación
Unconjugated
Grado
ExactAb™, Validated
SDS-PAGE
150 kDa
Método de purificación
Protein G purified
Concentración
See COA
Product Line
Productos biológicos
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes

CD14 Mouse mAb (Ab093874) - Western Blot
All lanes: CD14 Mouse mAb (Ab093874) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution

Predicted band size: 40 kDa
Observed band size: 51 kDa
Exposure time: 15.0 s

CD14 Mouse mAb (Ab093874) - Western Blot
All lanes: CD14 Mouse mAb (Ab093874) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/5000 dilution

Predicted band size: 40 kDa
Observed band size: 51 kDa
Exposure time: 7.8 s

CD14 Mouse mAb (Ab093874) - Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by CD14 Mouse mAb (Ab093874), and then analyzed by flow cytometry (1/100).

Aplicación
Aplicación
Dilution info
Flow Cytometry

1/100

WB

1/500 - 1/1000

Datos avanzados

Objetivos asociados (humanos)
CD14 Tbio Monocyte differentiation antigen CD14 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mecanismos de acción
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Preguntas frecuentes

What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Validated mean?
Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD14 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.

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