CD28 Mouse mAb (PE) - Primary antibody, specific to CD28, Mouse IgG1

Cat. No.: Ab185380

CALIDAD & PUREZA ExactAb™, Validated, Ex:565nm, Em:575nm, 5 μL/test

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

CD28.2

Conjugation

PE

Descripción general

Application:
Flow: 5 μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Especificaciones

Product Name

CD28 Mouse mAb (PE) - Primary antibody, specific to CD28, Mouse IgG1

Sinónimos

CD28 antigen (Tp44) | CD28 antigen | CD28 molecule | CD28 | MGC138290 | T-cell-specific surface glycoprotein CD28 | Tp44 | CD 28 antibody | CD28 antibody | CD28 antigen antibody | CD28 molecule antibody | CD28_HUMAN antibody | MGC138290 antibody | T cell

Especificaciones y pureza

ExactAb™, Validated, Ex:565nm, Em:575nm, 5 μL/test

Especies hospedadoras

Mouse

Especificidad

CD28

Inmunógeno

Human CD28 transfected cell line

Control positivo

Flow: Jurkat

Conjugación

PE

Excitación (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emisión (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Grado

Em:575nm, Ex:565nm, ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

5 μL/test

Product Line

Productos biológicos

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD28 Mouse mAb (PE) (Ab185380)

CD28 Mouse mAb (PE) (Ab185380) - Flow Cytometry
Flow cytometry analysis of CD28 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD28 Mouse mAb (PE) (Ab185380) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (PE) (Ab180308). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

Aplicación

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Objetivos asociados (humanos)

CD28 Tbio T-cell-specific surface glycoprotein CD28 (0 Activities)

Discover Target: CD28

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD28 Mouse mAb (PE)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.