GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.Ex:650nm?Fluorescence excitation maximum at 650 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal.Em:660nm?Fluorescence emission maximum at 660 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk.5μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume.
Specifications
Product Name
CD4 Rat mAb (APC) - Primary antibody, specific to CD4, Rat IgG2b
Specifications & Purity
Validated, Ex:650nm, Em:660nm, 5μL/test
Host species
Rat
Specificity
CD4
Immunogen
Mouse CTL clone V4
Positive Control
Flow: Murine splenocytes.
Conjugation
APC
Excitation (nm)
Red Laser(633nm)
Emission(nm)
660nm
Ex/Em(nm)
Ex:650nm;Em:660nm
Grade
Em:660nm, Ex:650nm, Validated
Isotype
Rat IgG2b
Purification Method
Protein G purified
Concentration
5μL/test
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
CD4 Rat mAb (APC) (Ab181112) - Flow Cytometry Flow cytometry analysis of CD4 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD4 Rat mAb (APC) (Ab181112) at 5μL/test for 1 hour at 4°C. Left panel - Rat IgG Isotype Control Antibody (APC) (Ab180313).
CD4 Rat mAb (APC) (Ab181112) - Flow Cytometry Flow cytometry analysis of CD4 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD4 Rat mAb (APC) (Ab181112) at 5μL/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (APC) (Ab180313). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Reviews
Customer Reviews
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