Carrier Free,Low Endotoxin,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE&HPLC),See COA Azide Free,Carrier Free,Low Endotoxin,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Mouse anti Human CD8 alpha Antibody, Monoclonal (UCHT4), could be used for Flow, WB, IP, IHC, IF, ICC, ELISA, Functional Assays and so on.
Application
Flow: ≤ 1.0µg/100w cells
WB: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
ELISA: Use at an assay dependent concentration.
Functional Assays: Use at an assay dependent concentration.
Protein Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
CD8 alpha Mouse mAb (Ab095360) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD8 alpha with CD8 alpha Mouse mAb (Ab095360) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150).
CD8 alpha Mouse mAb (Ab095360) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD8 alpha (red) with CD8 alpha Mouse mAb (Ab095360) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
CD8 alpha Mouse mAb (Ab095360) - Flow Cytometry
Flow Cytometry analysis of MOLT-4 cells labelling CD8 alpha (red) with CD8 alpha Mouse mAb (Ab095360) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
| Aplicación | Dilution info |
|---|---|
| Flow Cytometry | ≤1.0µg/100w cells |
| WB | Use at an assay dependent concentration. |
| Functional Assay | Use at an assay dependent concentration. |
| IP | Use at an assay dependent concentration. |
| IHC | Use at an assay dependent concentration. |
| IF/ICC | Use at an assay dependent concentration. |
| ELISA | Use at an assay dependent concentration. |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Carrier Free grade guide → View Validated grade guide → View Low Endotoxin grade guide → View Azide Free grade guide → View PBS Only grade guide →
