CD8 alpha Mouse mAb (Biotin) - Primary antibody, specific to CD8 alpha

Cat. No.: Ab190484

CALIDAD & PUREZA ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

OKT-8

Conjugation

Biotin

Descripción general

Mouse anti Human CD8 alpha Antibody, Monoclonal (OKT-8), could be used for Flow, in vivo CD8+ T cell depletion in humanized mice and so on.

Especificaciones

Product Name

CD8 alpha Mouse mAb (Biotin) - Primary antibody, specific to CD8 alpha

Sinónimos

CD8 | Leu2 | IMD116 | CD8alpha | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | Biotin Anti-Human CD8a Antibody[OKT-8] | Biotin Anti-Human CD8a (OKT8) | Biotin Mouse Anti-Human CD8 | CD8a Monoclonal Antibody (OKT-8), Biotin | Biotin anti-human

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Especies hospedadoras

Mouse

Especificidad

CD8 alpha

Inmunógeno

Human CD8α

Control positivo

Flow: Human peripheral blood lymphocytes

Conjugación

Biotin

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD8 alpha Mouse mAb (Biotin) (Ab190484)

CD8 alpha Mouse mAb (Biotin) (Ab190484) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Mouse mAb (Biotin) (Ab190484) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).

Flow Cytometry: CD8 alpha Mouse mAb (Biotin) (Ab190484)

CD8 alpha Mouse mAb (Biotin) (Ab190484) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Mouse mAb (Biotin) (Ab190484) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

Aplicación

Dilution info

Flow Cytometry

≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume

Objetivos asociados (humanos)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)