Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Rabbit anti Human Cdk4 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-2 µg/mL
IHC: 5-20 µg/mL
IF/ICC: 2.5 µg/mL
Protein Function
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Cdk4 Antibody (Ab095764) - Western Blot
All lanes: Cdk4 Antibody (Ab095764) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 34 kDa
Observed band size: 33kDa
Exposure time: 2.2 s
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Samples: Human Lymphoma Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Samples: Human Lung cancer Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Sample: Human Breast cancer Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Sample: Human Lymphoma Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Cdk4 Antibody (Ab095764) - IHC
DAB staining on IHC
Sample: Human Lung cancer Tissue
Primary Ab: 10 µg/mL Cdk4 Antibody (Ab095764)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| Aplicación | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| WB |
0.01-2 µg/mL
|
| IF/ICC |
2.5 µg/mL
|
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 ZJ23F1101529 | Certificate of Analysis | Nov 02, 2023 | Ab095764 |
| ZJ23F1101530 | Certificate of Analysis | Nov 02, 2023 | Ab095764 |
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View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide →
