CEBPA Mouse mAb - Primary antibody, specific to CEBPA, IgG1

Cat. No.: Ab095961

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0mg/mL

Application

ELISA, Flow Cytometry, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

4E10

Conjugation

Unconjugated

Descripción general

Mouse Monoclonal (4E10) to CEBPA , could be used for WB, Flow and ELISA.

Applications
WB：1/500 - 1/2000;
Flow：1/200 - 1/400;
ELISA ：1/10000;

Purity
Protein G purified

Function
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

Specifications

Product Name

CEBPA Mouse mAb - Primary antibody, specific to CEBPA, IgG1

Sinónimos

C/EBP alpha

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.0mg/mL

Especies hospedadoras

Mouse

Especificidad

CEBPA

Inmunógeno

Purified recombinant fragment of human CEBPA expressed in E. Coli.

Control positivo

WB: THP-1 cell lysate, Flow: HeLa cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

1.0mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C. Avoid freeze/thaw cycle.

Imágenes

Western Blot: CEBPA Mouse mAb (Ab095961)

CEBPA Mouse mAb (Ab095961) - Western Blot
All lanes: CEBPA Mouse mAb (Ab095961) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 42&26 kDa
Observed band size: 42&26 kDa

Flow Cytometry: CEBPA Mouse mAb (Ab095961)

CEBPA Mouse mAb (Ab095961) - Flow Cytometry
Flow cytometric analysis of HeLa cells using CEBPA Mouse mAb (Ab095961) at 1/200 dilution (green) and negative control (red).

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/200-1/400 ;

• ELISA:

  1/10000 ;

Objetivos asociados (humanos)

CEBPA Tbio CCAAT/enhancer-binding protein alpha (0 Activities)

Discover Target: CEBPA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)