COX IV Mouse mAb - Primary antibody, specific to COX4I1, Mouse IgG1

Cat. No.: Ab097414

GRADE & PURITY Validated, ExactAb™, 2.0 mg/mL

Application

Flow Cytometry, IF/ICC, IHC, IP, WB

Host species

Mouse

Reactive species

Goat, Hamster, Human, Monkey, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

4D11-B3-E8

Conjugation

Unconjugated

Descripción general

Mouse anti Human COX IV Antibody, Monoclonal antibody(4D11-B3-E8), could be used for WB, IHC, ICC, IF, Flow, IP and so on.

Application:

WB: 1/1000 

IHC: 1/50-1/200

ICC/IF: 1/100-1/200 

Flow: 1/100 

IP: 1/20 

Protein Function

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Specifications

Product Name

COX IV Mouse mAb - Primary antibody, specific to COX4I1, Mouse IgG1

Sinónimos

AL024441 antibody | COX 4 antibody | COX IV 1 antibody | COX IV antibody | COX IV-1 antibody | Cox4 antibody | COX41_HUMAN antibody | Cox4a antibody | COX4B antibody | COX4I1 antibody | COX4I2 antibody | COX4L2 antibody | COXIV antibody | Cytochrome c oxi

Especificaciones y pureza

ExactAb™, Validated, 2.0 mg/mL

Especies hospedadoras

Mouse

Especificidad

COX4I1

Inmunógeno

A synthetic peptide derived from human COX IV (AA 1-169).

Control positivo

WB: Jurkat, NIH/3T3, HeLa, C6, K562, MCF7, THP-1, Raji; IHC: Human colorectal carcinoma; ICC/IF: HeLa cells; IP: HeLa.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

Isotipo

Mouse IgG1

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

2.0 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: COX IV Mouse mAb (Ab097414)

COX IV Mouse mAb (Ab097414) - Western Blot
All lanes: COX IV Mouse mAb (Ab097414) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 5.0 s

ICC/IF: COX IV Mouse mAb (Ab097414)

COX IV Mouse mAb (Ab097414) - ICC/IF
Immunocytochemistry analysis of COX IV (green) in HeLa cells using COX IV Mouse mAb (Ab097414).

IHC: COX IV Mouse mAb (Ab097414)

COX IV Mouse mAb (Ab097414) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colorectal carcinoma using COX IV Mouse mAb (Ab097414). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IP: COX IV Mouse mAb (Ab097414)

COX IV Mouse mAb (Ab097414) - IP
Immunoprecipitation analysis of COX IV (4D11) in HeLa cell lysates using COX IV Mouse mAb (Ab097414)

Aplicación

• Aplicación: Dilution info
• IP:

  1/20;

• WB:

  1/1000 ;

• IHC:

  1/50-1/200;

• IF/ICC:

  1/100-1/200;

• Flow Cytometry:

  1/100;

Objetivos asociados (humanos)

COX4I1 Tbio Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (0 Activities)

Discover Target: COX4I1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)