GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human COX2 Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 0.01-5 µg/mL IHC: 5-20 µg/mL Protein Function Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
Specifications
Product Name
COX2 Antibody - Primary antibody, specific to COX2 / Cyclooxygenase 2, Rabbit IgG
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Especies hospedadoras
Rabbit
Especificidad
COX2 / Cyclooxygenase 2
Inmunógeno
Recombinant COX2 expressed in E.coli (AA 187-425)
Control positivo
WB: RAW264.7 treated with LPS cell lysates. IHC: Human liver, breast cancer, pancreatic cancer and kidney tissues. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Antigen affinity purified and Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
COX2 Antibody (Ab097432) - Western Blot All lanes: COX2 Antibody (Ab097432) at 1/3000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 74 kDa Observed band size: 71 kDa Exposure time: 0.5 s
COX2 Antibody (Ab097432) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 10 µg/mL COX2 Antibody (Ab097432) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
COX2 Antibody (Ab097432) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 10 µg/mL COX2 Antibody (Ab097432) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
COX2 Antibody (Ab097432) - IHC DAB staining on IHC Samples: Human Pancreatic cancer Tissue Primary Ab: 10 µg/mL COX2 Antibody (Ab097432) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
COX2 Antibody (Ab097432) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10 µg/mL COX2 Antibody (Ab097432) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
COX2 Antibody (Ab097432) - IF/ICC
IF analysis of COX2 Antibody (Ab097432) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with COX2 Antibody (Ab097432) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
IHC
5-20 µg/mL
WB
0.01-5 µg/mL
IF/ICC
2.5 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ24F0404251
