DUSP3 Antibody - Primary antibody, specific to DUSP3, Rabbit IgG

Cat. No.: Ab100596

GRADE & PURITY Validated, ExactAb™, 0.2 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human DUSP3 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein function

Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.

Specifications

Product Name

DUSP3 Antibody - Primary antibody, specific to DUSP3, Rabbit IgG

Sinónimos

dual specificity phosphatase 3 | dual specificity protein phosphatase 3 | Dual specificity protein phosphatase VHR | DUSP3 | EC 3.1.3.16 | EC 3.1.3.48 | Vaccinia H1-related phosphatase | vaccinia virus phosphatase VH1-related | VHR | VHRserine/threonine s

Especificaciones y pureza

ExactAb™, Validated, 0.2 mg/mL

Especies hospedadoras

Rabbit

Especificidad

DUSP3

Inmunógeno

A synthetic peptide derived from human DUSP3 (AA 1-17).

Control positivo

WB: HeLa, NIH/3T3, C6, Jurkat, MCF7, K562, HEK293, A431 and Hep G2 cell lysates. IHC: Human cholangiocarcinoma tissue. ICC/IF: HeLa cells.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

0.2 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: DUSP3 Antibody (Ab100596)

DUSP3 Antibody (Ab100596) - Western Blot
All lanes: DUSP3 Antibody (Ab100596) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 8.8 s

IF/ICC: DUSP3 Antibody (Ab100596)

DUSP3 Antibody (Ab100596) - IF/ICC
Immunocytochemistry analysis of DUSP3 (green) in HeLa using DUSP3 Antibody (Ab100596), and cells were counterstained with DAPI (blue).

IHC: DUSP3 Antibody (Ab100596)

DUSP3 Antibody (Ab100596) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using DUSP3 Antibody (Ab100596). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

• IHC:

  1/50 - 1/100;

Objetivos asociados (humanos)

DUSP3 Tchem Dual specificity protein phosphatase 3 (0 Activities)

Discover Target: DUSP3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción