ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Mouse monoclonal (7H12) to E Cadherin, could be used for WB, IHC and Flow.
Application
WB: 1/500-1/2000
IHC: 1/200-1/1000
Flow: 1/200-1/400
ELISA: 1/10000
Purity
Protein G purified.
Protein Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
E Cadherin Mouse mAb (Ab100725) - Western Blot
All lanes: E Cadherin Mouse mAb (Ab100725) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 135 kDa
Observed band size: 112&124 kDa
E Cadherin Mouse mAb (Ab100725) - IHC
Immunohistochemical analysis of paraffin-embedded Human gastric tissues using E Cadherin Mouse mAb (Ab100725) with DAB staining.
E Cadherin Mouse mAb (Ab100725) - IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using E Cadherin Mouse mAb (Ab100725) with DAB staining.
E Cadherin Mouse mAb (Ab100725) - Flow Cytometry
Flow cytometric analysis of HeLa cells using E Cadherin Mouse mAb (Ab100725) (green) and negative control (purple).
| Aplicación | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| Flow Cytometry |
1/200-1/400
|
| ELISA |
1/10000
|
| IHC |
1/200-1/1000
|
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 ZJ23F0700709 | Certificate of Analysis | Jul 26, 2023 | Ab100725 |
| ZJ23F0700712 | Certificate of Analysis | Jul 26, 2023 | Ab100725 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide →
