Estrogen Receptor beta Antibody - Primary antibody, specific to Estrogen Receptor beta, Rabbit IgG

Cat. No.: Ab102374

GRADE & PURITY Validated, ExactAb™, 0.6 mg/mL

Application

IHC, WB

Host species

Rabbit

Reactive species

Human

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human Estrogen Receptor beta Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

Specifications

Product Name

Estrogen Receptor beta Antibody - Primary antibody, specific to Estrogen Receptor beta, Rabbit IgG

Sinónimos

ER BETA antibody | ER-beta antibody | Erb antibody | ESR B antibody | ESR BETA antibody | ESR2 antibody | ESR2_HUMAN antibody | ESRB antibody | ESTRB antibody | estrogen nuclear receptor beta variant a antibody | estrogen nuclear receptor beta variant b a

Especificaciones y pureza

ExactAb™, Validated, 0.6 mg/mL

Especies hospedadoras

Rabbit

Especificidad

Estrogen Receptor beta

Inmunógeno

A synthetic peptide derived from human Estrogen Receptor beta (AA 480-530)

Control positivo

WB: MCF7, HeLa, Jurkat and K562 cell lysates. IHC: Human cervix cancer.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

Isotipo

Rabbit IgG

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

0.6 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Estrogen Receptor beta Antibody (Ab102374)

Estrogen Receptor beta Antibody (Ab102374) - Western Blot
All lanes: Estrogen Receptor beta Antibody (Ab102374) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 59 kDa
Observed band size: 55 kDa
Exposure time: 10.0 s

IHC: Estrogen Receptor beta Antibody (Ab102374)

Estrogen Receptor beta Antibody (Ab102374) - IHC
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer using Estrogen Receptor beta Antibody (Ab102374). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

Objetivos asociados (humanos)

ESR2 Tclin Estrogen receptor beta (0 Activities)

Discover Target: ESR2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

ESR2 Tclin Estrogen receptor beta (9272 Activities)

Discover Target: ESR2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de acción

Referencias

1. Junjie Luo, Zhiye Hu, Yuan Xiao, Tongxin Yang, Chune Dong, Jian Huang, Hai-Bing Zhou.  (2017)  Rational design and optimization of selenophenes with basic side chains as novel potent selective estrogen receptor modulators (SERMs) for breast cancer therapy.  MedChemComm,  8  (7): (1485-1497).  [PMID:30108860] [10.1039/C7MD00163K]