GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.See COA
Carrier Free, ExactAb™, Azide Free, Validated, See COA Azide Free,Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human FAK Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.5-2 µg/mL
IHC: 5-20 µg/mL
IF/ICC: 3.25 µg/mL
Protein Function
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Specifications
Product Name
FAK Antibody - Primary antibody, specific to FAK, Rabbit IgG
Carrier Free, ExactAb™, Azide Free, Validated, See COA
Especies hospedadoras
Rabbit
Especificidad
FAK
Inmunógeno
Recombinant FAK expressed in E.coli (AA 798-1041)
Control positivo
WB: C6, NIH/3T3, K562, HUVEC and MCF7 cell lysates. IHC: Human Liver tissue, Human Thyroid tissue, Human Liver cancer tissue. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Antigen affinity purified and Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
FAK Antibody (Ab102794) - Western Blot All lanes: FAK Antibody (Ab102794) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 125 kDa Observed band size: 111 kDa Exposure time: 15.6 s
FAK Antibody (Ab102794) - Western Blot All lanes: FAK Antibody (Ab102794) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 125 kDa Observed band size: 111 kDa Exposure time: 15.6 s
FAK Antibody (Ab102794) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 20 µg/mL FAK Antibody (Ab102794) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
FAK Antibody (Ab102794) - IHC DAB staining on IHC Samples: Human Thyroid Tissue Primary Ab: 20 µg/mL FAK Antibody (Ab102794) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
FAK Antibody (Ab102794) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 20 µg/mL FAK Antibody (Ab102794) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
FAK Antibody (Ab102794) - IF/ICC
IF analysis of FAK Antibody (Ab102794) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with FAK Antibody (Ab102794) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
Aplicación
Aplicación
Dilution info
IHC
5-20 µg/mL
WB
0.5-2 µg/mL
IF/ICC
3.25 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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