Ku80 Mouse mAb - Primary antibody, specific to XRCC5, Mouse IgG1

Cat. No.: Ab112438
Disponible para pedir
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 1.0 mg/mL
Application
ELISA, Flow Cytometry, IF/ICC, IHC, WB
Host species
Mouse
Reactive species
Human, Mouse
Isotype
Mouse IgG1
Clone Number
5C5
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μl
Ab112438-10μl
4
79,90US$
50μl
Ab112438-50μL
2
207,90US$
100μl
Ab112438-100μL
1
351,90US$
1ml
Ab112438-1mL
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
3.999,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Application:
WB: 1/500 - 1/2000. Detects a band of approximately 86 kDa (predicted molecular weight: 83 kDa).
IHC: 1/200 - 1/1000
IF/ICC: 1/200 - 1/1000
Flow: 1/200 - 1/400
ELISA: 1/10000


The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Specifications

Product Name
Ku80 Mouse mAb - Primary antibody, specific to XRCC5, Mouse IgG1
Sinónimos
86 kDa subunit of Ku antigen | ATP-dependent DNA helicase 2 subunit 2 | ATP-dependent DNA helicase II 80 kDa subunit | CTC box-binding factor 85 kDa subunit | CTC85 | CTCBF | DNA repair protein XRCC5 | EC 3.6.4.- | Ku80 | Ku86 | Lupus Ku autoantigen prote
Especificaciones y pureza
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Especies hospedadoras
Mouse
Especificidad
XRCC5
Inmunógeno
Purified recombinant fragment of human XRCC5 (corresponding to amino acids 61-340 of Human Ku80) expressed in E. Coli
Control positivo
WB: A549, HeLa, HEK293, K562 lysates. ICC/IF: HeLa cells. IHC: Human tonsil tissues, Human colon cancer tissue. Flow: HeLa cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Mouse IgG1
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein G purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid repeated freeze / thaw cycles.
Imágenes

Ku80 Mouse mAb (Ab112438) - Western Blot
All lanes: Ku80 Mouse mAb (Ab112438) at 1/1000 dilution


Samples: Lysates at 20 µg per lane


Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution


Predicted band size: 83 kDa
Observed band size: 83 kDa

Ku80 Mouse mAb (Ab112438) - ICC/IF
IF analysis of HeLa cells using Ku80 Mouse mAb (Ab112438) at a dilution of 1/200 (green). Red: Actin filaments have been labeled with AF555 phalloidin.

Ku80 Mouse mAb (Ab112438) - IHC
Immunohistochemical analysis of paraffin-embedded Human tonsil tissues using Ku80 Mouse mAb (Ab112438) at a dilution of 1/200 with DAB staining.

Ku80 Mouse mAb (Ab112438) - IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using Ku80 Mouse mAb (Ab112438) at a dilution of 1/200 with DAB staining.

Ku80 Mouse mAb (Ab112438) - Flow Cytometry
Flow cytometric analysis of HeLa cells using Ku80 Mouse mAb (Ab112438) at a dilution of 1/200 (green) and negative control (purple).

Aplicación
AplicaciónDilution info
WB

1/500-1/2000

IF/ICC

1/200-1/1000

Flow Cytometry

1/200-1/400

ELISA

1/10000

IHC

1/200-1/1000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
XRCC5 Tbio X-ray repair cross-complementing protein 5 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeFechaArticulo
ZJ23F0700540Certificate of AnalysisApr 09, 2026 Ab112438
ZJ23F0700541Certificate of AnalysisApr 09, 2026 Ab112438
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide →

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