MADH7/SMAD7 Antibody - Primary antibody, specific to SMAD7, Rabbit IgG

Cat. No.: Ab113970

GRADE & PURITY Validated, 1.0 mg/mL

Application

WB

Host species

Rabbit

Reactive species

Goat, Human, Mouse, Rat, Sheep

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human MADH7/SMAD7 Antibody, Polyclonal, could be used for WB and so on.
Application
WB: 1/500-1/2000
Protein Function
Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Specifications

Product Name

MADH7/SMAD7 Antibody - Primary antibody, specific to SMAD7, Rabbit IgG

Sinónimos

CRCS3 | FLJ16482 | hSMAD7 | MAD homolog 7 | MAD homolog 8 | MAD, mothers against decapentaplegic homolog 7 (Drosophila) | MADH7 | MADH7mothers against decapentaplegic homolog 7 | MADH8 | Mothers against decapentaplegic homolog 8 | Mothers against decapent

Especificaciones y pureza

Validated, 1.0 mg/mL

Especies hospedadoras

Rabbit

Especificidad

SMAD7

Inmunógeno

A synthetic peptide derived from human SMAD7.

Control positivo

WB: Mouse lung, Jurkat, NIH/3T3 and C6 lysates.

Conjugación

Unconjugated

Grado

Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Immunogen affinity purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: MADH7/SMAD7 Antibody (Ab113970)

MADH7/SMAD7 Antibody (Ab113970) - Western Blot
All lanes: MADH7/SMAD7 Antibody (Ab113970) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 42 kDa
Exposure time: 21.3 s

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/2000;

Objetivos asociados (humanos)

SMAD7 Tbio Mothers against decapentaplegic homolog 7 (0 Activities)

Discover Target: SMAD7

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)