GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.6 mg/mL
ExactAb™, Validated, Carrier Free, 0.6 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human MAPK11 Antibody, Polyclonal antibody, could be used for WB, IHC and so on. Application WB: 1/500 - 1/1000 IHC: 1/50 - 1/100 Protein Function Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2. Post-translational Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Specifications
Product Name
MAPK11 Antibody - Primary antibody, specific to MAPK11, Rabbit IgG
A synthetic peptide derived from human MAPK11. (AA 251-364).
Control positivo
WB: HeLa, MCF7 and Jurkat cell lysates. IHC: Human brain tissue. IF/ICC: HeLa cells
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
0.6 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
MAPK11 Antibody (Ab119724) - Western Blot All lanes: MAPK11 Antibody (Ab119724) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 60.0 s
MAPK11 Antibody (Ab119724) - Western Blot All lanes: MAPK11 Antibody (Ab119724) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 39 kDa Exposure time: 100.0 s
MAPK11 Antibody (Ab119724) - IHC Immunohistochemistry analysis of paraffin-embedded Human brain using MAPK11 Antibody (Ab119724). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
MAPK11 Antibody (Ab119724) - IF/ICC
IF analysis of MAPK11 Antibody (Ab119724) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MAPK11 Antibody (Ab119724) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
IF/ICC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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