GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.See COA
Carrier Free, ExactAb™, Azide Free, Validated, See COA Azide Free,Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human MGMT Antibody, Polyclonal antibody, could be used for WB, IHC and so on. Application WB: 0.5-3µg/mL IHC: 5-30µg/mL Protein Function Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Specifications
Product Name
MGMT Antibody - Primary antibody, specific to MGMT, Rabbit IgG
Sinónimos
6 O methylguanine DNA methyltransferase antibody | 6-O-methylguanine-DNA methyltransferase antibody | Agat antibody | AGT antibody | AI267024 antibody | EC 2.1.1.63 antibody | Methylated DNA protein cysteine methyltransferase antibody | Methylated-DNA--pr
Especificaciones y pureza
Carrier Free, ExactAb™, Azide Free, Validated, See COA
Especies hospedadoras
Rabbit
Especificidad
MGMT
Inmunógeno
Recombinant MGMT expressed in E.coli (AA 1-207).
Control positivo
WB: MCF7, Jurkat, HeLa, MOLT-4, HT-29 and Hep G2 cell lysate. IHC: Human stomach tissue.
Conjugación
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
MGMT Antibody (Ab115239) - Western Blot All lanes: MGMT Antibody (Ab115239) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 21 kDa Observed band size: 24 kDa Exposure time: 2.6 s
MGMT Antibody (Ab115239) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 10 µg/mL MGMT Antibody (Ab115239) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Aplicación
Aplicación
Dilution info
IHC
5-30 µg/mL
WB
0.5-3 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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