MHC class II I E kappa Mouse mAb - Primary antibody, specific to MHC class II I E kappa, Mouse IgG2a

Cat. No.: Ab115290

GRADE & PURITY Carrier Free, Validated, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay

Host species

Mouse

Reactive species

Mouse, Rat

Isotype

Mouse IgG2a

Clone Number

14-4-4S

Conjugation

Unconjugated

Descripción general

Mouse anti Mouse/Rat MHC class II I E kappa Antibody, Monoclonal (14-4-4S), could be used for Flow, in vivo blocking of antigen presentation and so on.
Application
Flow: Use at an assay dependent concentration.
in vivo blocking of antigen presentation: Use at an assay dependent concentration.

Specifications

Product Name

MHC class II I E kappa Mouse mAb - Primary antibody, specific to MHC class II I E kappa, Mouse IgG2a

Sinónimos

Eb | Ia4 | H2Eb | Ia-4 | H-2Eb | H2 Ea antibody | H2-Ea antibody | Eb | Ia4 | H2Eb | Ia-4 | H-2Eb | H2 Ea antibody | H2-Ea antibody

Especificaciones y pureza

Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Especies hospedadoras

Mouse

Especificidad

MHC class II I E kappa

Inmunógeno

C3H mouse skin graft and spleen cells

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, PBS Only, Validated

Isotipo

Mouse IgG2a

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Imágenes

Flow Cytometry: MHC class II I E kappa Mouse mAb (Ab115290)

MHC class II I E kappa Mouse mAb (Ab115290) - Flow Cytometry
Flow Cytometry analysis of rat splenocytes cells labelling MHC class II I E kappa with MHC class II I E kappa Mouse mAb (Ab115290) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: MHC class II I E kappa Mouse mAb (Ab115290)

MHC class II I E kappa Mouse mAb (Ab115290) - Flow Cytometry
Flow Cytometry analysis of rat splenocytes cells labelling MHC class II I E kappa (red) with MHC class II I E kappa Mouse mAb (Ab115290) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo blocking of antigen presentation:

  Use at an assay dependent concentration.;

Objetivos asociados (humanos)

HLA-DRB5 Tbio HLA class II histocompatibility antigen, DR beta 5 chain (0 Activities)

Discover Target: HLA-DRB5

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)