Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,0.5 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Application
WB: 1/1,000-1/10,000 ; Predicted molecular weight: 29 kDa
Flow: 0.032 μg/mL - 1.0 μg/mL
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.
PCNA Mouse mAb (Ab181179) - Western Blot
All lanes: PCNA Antibody (Ab181179) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 29.0 kDa
Observed band size: 31.0 kDa
Exposure time: 54.2 s
PCNA Mouse mAb (Ab181179) - Flow Cytometry
Overlay histogram showing HeLa cells stained with PCNA Mouse mAb (Ab181179) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
| Aplicación | Dilution info |
|---|---|
| WB |
1/1,000-1/10,000 ; Predicted molecular weight: 29 kDa.
|
| Flow Cytometry | 0.032 μg/mL - 1.0 μg/mL |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 ZJ24F0102680 | Certificate of Analysis | Dec 16, 2025 | Ab181179 |
| ZJ24F0102681 | Certificate of Analysis | Dec 16, 2025 | Ab181179 |
| ZJ24F0102682 | Certificate of Analysis | Dec 16, 2025 | Ab181179 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →
