GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.1.14 mg/mL
ExactAb™, Validated, Carrier Free, High performance, 1.14 mg/mL Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human PPP2R1A Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/3000 IHC: 1/50-1/100 Protein Function The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.
Specifications
Product Name
PPP2R1A Antibody - Primary antibody, specific to PPP2R1A, Rabbit IgG
Sinónimos
2AAA_HUMAN antibody | 6330556D22Rik antibody | Alpha isoform of regulatory subunit A, protein phosphatase antibody | DKFZp470L0914 antibody | Medium tumor antigen-associated 61 kDa protein antibody | MGC128399 antibody | MGC786 antibody | MGC83000 antibod
Especificaciones y pureza
ExactAb™, Validated, Carrier Free, High performance, 1.14 mg/mL
Especies hospedadoras
Rabbit
Especificidad
PPP2R1A
Inmunógeno
Fusion protein of human PPP2R1A (AA 500-580)
Control positivo
WB: C6, Jurkat and HEK293 cell lysates. IHC: Human liver cancer lysates. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
1.14 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
PPP2R1A Antibody (Ab122840) - Western Blot All lanes: PPP2R1A Antibody (Ab122840) at 1/3000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 65 kDa Observed band size: 60 kDa Exposure time: 5.0 s
PPP2R1A Antibody (Ab122840) - IHC Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PPP2R1A Antibody (Ab122840). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
PPP2R1A Antibody (Ab122840) - IF/ICC
IF analysis of PPP2R1A Antibody (Ab122840) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PPP2R1A Antibody (Ab122840) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
IHC
1/50 - 1/100
WB
1/500-1/3000
IF/ICC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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