GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.8 mg/mL
ExactAb™, validado, sin portador, 0,8 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Conservar a -20°C,Evitar la congelación y descongelación repetidas Ships Hielera + almohadillas de hielo Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Anticuerpo de conejo anti PSMF1 humano, anticuerpo policlonal, se puede utilizar para WB, IHC, etc. Aplicación WB: 1/500 - 1/1000 IHC: 1/50-1/100 Función de la proteína Desempeña un papel importante en el control de la función del proteasoma. Inhibe la hidrólisis de sustratos proteicos y peptídicos por el proteasoma 20S. También inhibe la activación del proteasoma por las proteínas reguladoras del proteasoma PA700 y PA28
Specifications
Product Name
Anticuerpo PSMF1 - Primary antibody, specific to PSMF1, Rabbit IgG
WB: LNCaP, K562 and HEK293 cell lysates. IHC: Human liver cancer tissue. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
0.8 mg/mL
Almacenamiento y envío
Forma
Líquido
Condiciones de almacenamiento de almacenamiento
Conservar a -20°C,Evitar la congelación y descongelación repetidas
Enviado en
Hielera + almohadillas de hielo
Estabilidad y almacenamiento
Conservar a 4°C a corto plazo (1-2 semanas). Conservar a -20°C a largo plazo (24 meses). Tras la recepción, se recomienda realizar alícuotas. Evitar el ciclo de congelación/descongelación.
Imágenes
PSMF1 Antibody (Ab123731) - Western Blot All lanes: PSMF1 Antibody (Ab123731) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 30 kDa Observed band size: 31 kDa Exposure time: 11.0 s
PSMF1 Antibody (Ab123731) - IHC Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PSMF1 Antibody (Ab123731). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
PSMF1 Antibody (Ab123731) - IF/ICC IF analysis of PSMF1 Antibody (Ab123731) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PSMF1 Antibody (Ab123731) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
IF/ICC
1/200
WB
1/500-1/1000
IHC
1/50-1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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